您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say)

文献类型： 外文期刊

作者： Fu, Kai-Yun 1 ; Lu, Feng-Gong 1 ; Guo, Wen-Chao 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Educ Minist Key Lab Integrated Management Crop Di, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi, Peoples R China

关键词： Leptinotarsa decemlineata;juvenile hormone diol kinase;RNA interference;juvenile hormone titer;performance

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Juvenile hormone diol kinase (JHDK) is an enzyme involved in JH degradation. In the present article, a putative JHDK cDNA (LdJHDK) was cloned from the Colorado potato beetle Leptinotarsa decemlineata. The cDNA consists of 814 bp, containing a 555 bp open reading frame encoding a 184 amino acid protein. LdJHDK reveals a high degree of identity to the previously reported insect JHDKs. It possesses three conserved purine nucleotide-binding elements, and contains three EF-hand motifs (helix-loop-helix structural domains). LdJHDK mRNA was mainly detected in hindgut and Malpighian tubules. Besides, a trace amount of LdJHDK mRNA was also found in thoracic muscles, brain-corpora cardiaca-corpora allata complex, foregut, midgut, ventral ganglia, fat body, epidermis, and hemocytes. Moreover, LdJHDK was expressed throughout all developmental stages. Within the first, second, and third larval instar, the expression levels of LdJHDK were higher just before and right after the molt, and were lower in the intermediate instar. In the fourth larval instar, the highest peak of LdJHDK occurred 56 h after ecdysis. Ingestion of double-stranded RNA (dsRNA) against LdJHDK successfully knocked down the target gene, increased JH titer, and significantly upregulated LdKr-h1 mRNA level. Knockdown of LdJHDK significantly impaired adult emergence. Thus, we provide a line of experimental evidence in L. decemlineata to support that LdJHDK encodes function protein involved in JH degradation. (C) 2015 Wiley Periodicals, Inc.

  • 相关文献

[1]Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle. Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao.

[2]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

[3]Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Fu, Kai-Yun,Li, Qian,Zhou, Li-Tao,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[4]RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Mu, Li-Li,Chen, Xu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[5]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[6]Knocking down a putative Delta(1)-pyrroline-5-carboxylate dehydrogenase gene by RNA interference inhibits flight and causes adult lethality in the Colorado potato beetle Leptinotarsa decemlineata (Say). Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Wang, Xin-Xin,Li, Guo-Qing,Wan, Pin-Jun,Guo, Wen-Chao.

[7]Identification of ten mevalonate enzyme-encoding genes and their expression in response to juvenile hormone levels in Leptinotarsa decemlineata (Say). Li, Qian,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[8]The subunit gene Ld alpha 1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata. Qu, Yang,Chen, Jinhua,Li, Chenge,Wang, Qiang,Han, Zhaojun,Jiang, Weihua,Guo, Wenchao.

[9]Physiological roles of trehalose in Leptinotarsa larvae revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes. Shi, Ji-Feng,Xu, Qing-Yu,Sun, Qiang-Kun,Meng, Qing-Wei,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[10]The P450 enzyme Shade mediates the hydroxylation of ecdysone to 20-hydroxyecdysone in the Colorado potato beetle, Leptinotarsa decemlineata. Kong, Y.,Liu, X-P.,Wan, P-J.,Shi, X-Q.,Li, G-Q.,Guo, W-C..

[11]Novaluron ingestion causes larval lethality and inhibits chitin content in Leptinotarsa decemlineata fourth-instar larvae. Xu, Qing-Yu,Meng, Qing-Wei,Shi, Ji-Feng,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao. 2017

[12]Transcription changes of a putative trehalose-6-phosphate synthase gene in response to hormone stimulation in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Xu, Qing-Yu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[13]RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Zhou, Li-Tao,Jia, Shuang,Wan, Pin-Jun,Kong, Ye,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[14]Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say). Shi, Xiao-qin,Xiong, Man-Hui,Jiang, Wei-Hua,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun. 2012

[15]A putative Delta(1)-pyrroline-5-carboxylate synthetase involved in the biosynthesis of proline and arginine in Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[16]Effect of Temperature on the Occurrence and Distribution of Colorado Potato Beetle (Coleoptera: Chrysomelidae) in China. Liu, Huai,Wang, Jin-Jun,Li, Chao,Cheng, Deng-Fa,Zhang, Yun-Hui,Sun, Jin-Rui,Huang, Fangneng,Guo, Wen-Chao. 2014

[17]Receptor tyrosine kinase genes respond transcriptionally to sublethal doses of five insecticides by a mode-of-action independent way in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Zhu, Tao-Tao,Li, Guo-Qing,Guo, Wen-Chao. 2016

[18]The basic helix-loop-helix transcription factors in the Colorado potato beetle Leptinotarsa decemlineata. Fu, Kai-Yun,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2015

[19]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

[20]Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference. Guo, Wen-Chao,Liu, Xin-Ping,Fu, Kai-Yun,Shi, Ji-Feng,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台