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Superior fluorescent probe for detection of potassium ion

文献类型： 外文期刊

作者： Chen, Zhengbo 1 ; Tan, Lulu 1 ; Hu, Liangyu 1 ; Luan, Yunxia 2 ;

作者机构： 1.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

2.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

关键词： Fluorescence;Aptamer;Potassium ion;PicoGreen dye

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

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收录情况： SCI

摘要： Here, a simple, and highly sensitive fluorescent assay is designed to monitor K+. The versatile, robust biosensing strategy is based on the specific recognition utility of label-free aptamers with their targets and PicoGreen dye as the signal probe. The aptamers undergo a conformational change to a secondary structure such as G-quadruplex in the presence of targets. In addition to a conformational change with its targets, the remaining single-stranded DNA (ssDNA) aptamer form a duplex structure with its complete complementary sequence. Conformational changes of aptamers as well as fluorescence amplification produce clear signal-off in the presence of targets. Fluorescent assay employing this mechanism for the detection of K+ is highly sensitive, and selective. The detection limit of the K+ assay is determined to be 2.37 pM. The sensing strategy is low-cost and simple in its operation without requirement for complex labeling of probe DNA or sophisticated synthesis of the fluorescent compound. Also, the method has less structural requirement of complexes of aptamers with their targets, thus rending its wilder applications for various targets. (C) 2015 Elsevier B.V. All rights reserved.

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