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Degradation and detection of transgenic Bacillus thuringiensis DNA and proteins in flour of three genetically modified rice events submitted to a set of thermal processes

文献类型： 外文期刊

作者： Wang, Xiaofu 1 ; Chen, Xiaoyun 1 ; Xu, Junfeng 1 ; Dai, Chen 2 ; Shen, Wenbiao 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agr Qual & Standard Agroprod, Hangzhou 310021, Zhejiang, Peoples R China

2.Nanjing Agr Univ, Lab Ctr Life Sci, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

关键词： Genetically modified;Insect-resistant rice;DNA degradation;Protein degradation;Thermal processing;GMO detection

期刊名称：FOOD AND CHEMICAL TOXICOLOGY （ 影响因子：6.023； 五年影响因子：5.844 ）

ISSN：

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收录情况： SCI

摘要： This study aimed to investigate the degradation of three transgenic Bacillus thuringiensis (Bt) genes (Cry1Ab, Cry1Ac, and Cry1Ab/Ac) and the corresponding encoded Bt proteins in KMD1, KF6, and TT51-1 rice powder, respectively, following autoclaving, cooking, baking, or microwaving. Exogenous Bt genes were more stable than the endogenous sucrose phosphate synthase (SPS) gene, and short DNA fragments were detected more frequently than long DNA fragments in both the Bt and SPS genes. Autoclaving, cooking (boiling in water, 30 min), and baking (200 degrees C, 30 min) induced the most severe Bt protein degradation effects, and Cry1Ab protein was more stable than Cry1Ac and Cry1Ab/Ac protein, which was further confirmed by baking samples at 180 degrees C for different periods of time. Microwaving induced mild degradation of the Bt and SPS genes, and Bt proteins, whereas baking (180 degrees C, 15 min), cooking and autoclaving led to further degradation, and baking (200 degrees C, 30 min) induced the most severe degradation. The findings of the study indicated that degradation of the Bt genes and proteins somewhat correlated with the treatment intensity. Polymerase chain reaction, enzyme-linked immunosorbent assay, and lateral flow tests were used to detect the corresponding transgenic components. Strategies for detecting transgenic ingredients in highly processed foods are discussed. (C) 2015 Elsevier Ltd. All rights reserved.

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[1]Endpoint Visual Detection of Three Genetically Modified Rice Events by Loop-Mediated Isothermal Amplification. Chen, Xiaoyun,Wang, Xiaofu,Zhou, Yu,Huang, Sainan,Miao, Qingmei,Zhu, Qing,Xu, Junfeng,Jin, Nuo,Huang, Sainan. 2012

[2]Targeting the middle region of CP4-EPSPS protein for its traceability in highly processed soy-related products. Wu, Honghong,Zhang, Yihua,He, Jian,Shen, Wenbiao,Wang, Xiaofu,Zhou, Xinghu,Huang, Ming,Wu, Honghong,Zhou, Xinghu.

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