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Comparative proteomic analysis of floral color variegation in peach

文献类型： 外文期刊

作者： Zhou, Yong 1 ; Wu, Xinxin 1 ; Zhang, Zhen 1 ; Gao, Zhihong 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

关键词： Comparative proteoms;Ornamental peach;Variegation;Pigment

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

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收录情况： SCI

摘要： Variegation in flower is a special trait in ornamental peach (Prunus persica L.). To investigate the mechanism of color variegation, we used a combination of two dimensional gel electrophoresis and mass spectrometry to explore the proteomic profiles between variegated flower (VF) and red flower (RF) buds of the peach cultivar 'Sahong Tao'. More than 500 highly reproducible protein spots (P < 0.05) were detected and 72 protein spots showed a greater than two-fold difference in their values. We identified 70 proteins that may play roles in petal coloration. The mRNA levels of the corresponding genes were detected using quantitative RT-PCR. The results show that most of the proteins are involved in energy and metabolism, which provide energy and substrates. We found that LDOX, WD40, ACC, and PPO II are related to the pigment biosynthetic pathway. The activity of PPO enzyme was further validated and showed the higher with significant differences in RF compared with the VF ones. Moreover, the four UCH proteins are involved in protein fate and may be important in post-translational modifications in peach flowers. Our study is the first comparative proteomic analysis of floral variegation and will contribute to further investigations into the molecular mechanism of flower petal coloration in ornamental peach. (C) 2015 Elsevier Inc. All rights reserved.

  • 相关文献

[1]UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot. Wu, Xinxin,Ni, Xiaopeng,Zhou, Yong,Gao, Zhihong,Wu, Xinxin,Gong, Qinghua. 2017

[2]Development of Ty1-copia retrotransposon-based SSAP molecular markers for the study of genetic diversity in peach. Jiao, Yun,Ma, Rui-juan,Shen, Zhi-jun,Yu, Ming-liang.

[3]Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy. Han, J.,Wang, W. Y.,Leng, X. P.,Jiang, W. B.,Guo, L.,Yu, M. L.,Ma, R. J.. 2014

[4]Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum Thunb. ex Murray leaves. Li, S-S.,Li, Q-Z.,Tang, L.,Wen, J.. 2017

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