Designed diblock hairpin probes for the nonenzymatic and label-free detection of nucleic acid
文献类型： 外文期刊
作者： Wen, Junlin 1 ; Chen, Junhua 2 ; Zhuang, Li 2 ; Zhou, Shungui 2 ;
作者机构： 1.Chinese Acad Sci, Guangzhou Inst Geochem, Beijing 100864, Peoples R China
2.Guangdong Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Agr Environm Pollut Integrated, Guangzhou 510650, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
关键词： Colorimetric assay;Branched DNA junction;Gold nanoparticles;Catalytic hairpin assembly;Shewanella oneidensis
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The detection of nucleic acid sequences is of great importance in a variety of fields. An ultrasensitive DNA sensing platform is constructed using elaborately designed diblock hairpin probes (DHPs) that are composed of hairpin and poly-adenine blocks. The introduction of an initiator DNA target triggers the catalytic assembly of probes DHP1, DHP2 and DHP3 to fabricate numerous poly-adenine-tailed branched DNA junctions, which significantly amplify the signal of the target-DNA-recognizing event without any enzyme. Coupled to a gold nanoparticle-based colorimetric assay, the amplified recognition signal can be quantitatively detected or visually read with the naked eye. The combination of the high-efficiency target-catalyzed DHP assembly and sensitive gold-based colorimetric assay offers an ultrasensitive detection of DNA with a detection limit of 0.1 pM and a dynamic range from 0.01 to 5 pM. The proposed sensing platform can discriminate even single-base mutations. Moreover, the sensing platform can be expanded to detect pollutant-degrading-bacteria-specific DNA sequences. The proposed sensing system should offer an alternative approach for the detection of nucleic acids in the fields of microbiology, biogeochemistry, and environmental sciences. (C) 2016 Published by Elsevier B.V.
- 相关文献
作者其他论文 更多>>
-
Heavy Metal-Induced Assembly of DNA Network Biosensor from Double-Loop Hairpin Probes for Ultrasensitive Detection of UO2+2 ° in Water and Soil Samples
作者：Wang, Xu;Guo, Junhui;Chen, Junhua;Lv, Yiwen;Chen, Manjia;Tong, Hui;Chen, Junhua;Liu, Chengshuai
关键词：
-
Intelligent monitoring of the available lead (Pb) and cadmium (Cd) in soil samples based on half adder and half subtractor molecular logic gates
作者：Chen, Junhua;Liu, Chengshuai;Chen, Junhua;Lv, Yiwen;Chen, Manjia;Tong, Hui;Wang, Xu
关键词：Molecular logic gate; Intelligent sensing; DNA recognition; Heavy metal; Available Pb; Available Cd
-
Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures
作者：Tang, Jia;Zhuang, Li;Ma, Jinlian;Tang, Ziyang;Yu, Zhen;Zhou, Shungui
关键词：
-
Effect of ferrihydrite biomineralization on methanogenesis in an anaerobic incubation from paddy soil
作者：Zhuang, Li;Xu, Jielong;Tang, Jia;Zhou, Shungui
关键词：ferrihydrite mineralization;methanogenesis;magnetite;direct electric syntrophy;Geobacter
-
Facile Synthesis of MnO2/Polypyrrole/MnO2 Multiwalled Nanotubes as Advanced Electrocatalysts for the Oxygen Reduction Reaction
作者：Yuan, Haoran;Deng, Lifang;Chen, Yong;Tang, Jiahuan;Zhou, Shungui;Yuan, Yong;Yuan, Haoran;Deng, Lifang;Chen, Yong
关键词：hydrothermal synthesis;microbial fuel cells;MnO2/Polypyrrole/MnO2;nanotubes;oxygen reduction reaction
-
Genome sequence of a dissimilatory Fe(III)-reducing bacterium Geobacter soli type strain GSS01(T)
作者：Yang, Guiqin;Yang, Guiqin;Chen, Shanshan;Zhou, Shungui;Yang, Guiqin;Liu, Yongfeng
关键词：Geobacter soli;Extracellular electron transfer;Insoluble Fe(III) oxides reduction;Cytochrome;Pilin protein
-
Axial Ligation of Heme in c-Type Cytochromes of Living Shewanella oneidensis: A New Insight into Enhanced Extracellular Electron Transfer
作者：Yuan, Yong;Zhou, Shungui;Li, Laicai
关键词：axial ligation;c-type cytochromes;density functional calculations;extracellular electron transfer;Shewanella oneidensis
