Heavy Metal-Induced Assembly of DNA Network Biosensor from Double-Loop Hairpin Probes for Ultrasensitive Detection of UO2+2 ° in Water and Soil Samples
文献类型： 外文期刊
作者： Chen, Junhua 3 ; Wang, Xu 1 ; Guo, Junhui 2 ; Lv, Yiwen 3 ; Chen, Manjia 3 ; Tong, Hui 3 ; Liu, Chengshuai 4 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Qual Stand & Monitoring Technol Agroprod, Guangzhou 510640, Peoples R China
2.Jiangmen Polytech, Sch Mat & Food, Jiangmen 529000, Peoples R China
3.Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Peoples R China
4.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China
期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：7.4； 五年影响因子：7.0 ）
ISSN： 0003-2700
年卷期： 2024 年 96 卷 7 期
页码：
收录情况： SCI
摘要： The uranyl ion (UO22+) is the most stable form of uranium, which exhibits high toxicity and bioavailability posing a severe risk to human health. The construction of ultrasensitive, reliable, and robust sensing techniques for UO22+ detection in water and soil samples remains a challenge. Herein, a DNA network biosensor was fabricated for UO22+ detection using DNAzyme as the heavy metal recognition element and double-loop hairpin probes as DNA assembly materials. UO22+-activated specific cleavage of the DNAzyme will liberate the triggered DNA fragment, which can be utilized to launch a double-loop hairpin probe assembly among H-ab, H-bc, and H-ca. Through multiple cyclic cross-hybridization reactions, hexagonal DNA duplex nanostructures (n[H-ab center dot H-bc center dot H-ca]) were formed. This DNA network sensing system generates a high fluorescence response for UO22+ monitoring. The biosensor is ultrasensitive, with a detection limit of 2 pM. This sensing system also displays an excellent selectivity and robustness, enabling the DNA network biosensor to work even in complex water and soil samples with excellent accuracy and reliability. With the advantages of enzyme-free operation, outstanding specificity, and high sensitivity, our proposed DNA network biosensor provides a reliable, simple, and robust method for trace levels of UO22+ detection in environmental samples.
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