您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Two Leptinotarsa uridine diphosphate N-acetylglucosamine pyrophosphorylases are specialized for chitin synthesis in larval epidermal cuticle and midgut peritrophic matrix

文献类型： 外文期刊

作者： Shi, Ji-Feng 1 ; Fu, Jia 1 ; Mu, Li-Li 1 ; Guo, Wen-Chao 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

关键词： Uridine diphosphate-N-acetylglucosamine-pyrophosphorylase;Chitin;Ecdysis;Hormone

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Uridine diphosphate-N-acetylglucosamine-pyrophosphorylase (UAP) is involved in the biosynthesis of chitin, an essential component of the epidermal cuticle and midgut peritrophic matrix (PM) in insects. In the present paper, two putative LdUAP genes were cloned in Leptinotarsa decemlineata. In vivo bioassay revealed that 20-hydroxyecdysone (20E) and an ecdysteroid agonist halofenozide activated the expression of the two LdUAPs, whereas a decrease in 20E by RNA interference (RNAi) of an ecdyster-oidogenesis gene LdSHD and a 20E signaling gene LdF7Z-F1 repressed the expression. Juvenile hormone OH), a JH analog pyriproxyfen and an increase in JH by RNAi of an allatostatin gene LdAS-C down regulated LdUAP1 but upregulated LdUAP2, whereas a decrease in JH by silencing of a JH biosynthesis gene LdJHAMT had converse effects. Thus, expression of LdUAPs responded to both 20E and JH. Moreover, knockdown of LdUAP1 reduced chitin contents in whole larvae and integument samples, thinned tracheal taenidia, impaired larval larval molt, larval-pupal ecdysis and adult emergence. In contrast, silencing of LdUAP2 significantly reduced foliage consumption, decreased chitin content in midgut samples, damaged PM, and retarded larval growth. The resulting larvae had lighter fresh weights, smaller body sizes and depleted fat body. As a result, the development was arrested. Combined knockdown of LdUAP1 and LdUAP2 caused an additive negative effect. Our data suggest that LdUAP1 and LdUAP2 have specialized functions in biosynthesizing chitin in the epidermal cuticle and PM respectively in L. decemlineata. (C) 2015 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Effects of nitric oxide treatment on the cell wall softening related enzymes and several hormones of papaya fruit during storage. Guo, Qin,Chen, Weixin,Li, Xueping,Guo, Qin,Zhang, Yuli,Wang, Jide,Wu, Bin. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台