Label-free DNA Y junction for bisphenol A monitoring using exonuclease III-based signal protection strategy
文献类型： 外文期刊
作者： Chen, Junhua 1 ; Zhou, Shungui 1 ;
作者机构： 1.Guangdong Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Agr Environm Pollut Integrated, Guangzhou 510650, Guangdong, Peoples R China
关键词： DNA Y junction;Exonuclease III;Label-free;Bisphenol A;Signal amplification
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）
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收录情况： SCI
摘要： A label-free DNA Y junction sensing platform for the amplified detection of bisphenol A (BPA) has been constructed by the ingenious combination of toehold-mediated strand displacement and exonuclease III (Exo III)-based signal protection strategy. Three hairpin probes were utilized as the building blocks to fabricate the DNA Y junction with cascaded signal amplification via a series of toehold-mediated strand displacement reactions. Exo III was employed as a protecting agent for the first time to keep the Y-shaped molecular architecture intact, thereby greatly enhancing the fluorescence intensity of DNA intercalator SYBR Green I. The resulting biosensor exhibits ultrasensitivity towards BPA at low concentration (5 fM) without any labeling, modification, immobilization, or washing procedure. Our proposed sensing system also displays remarkable specificity to BPA against other possible interference molecules. Moreover, this DNA junction biosensor is robust and can be applied to the reliable monitoring of spiked BPA in environmental water samples with good recovery and accuracy. With the successful demonstration for BPA detection, the label-free DNA Y junction can be readily expanded to monitor other analytes in a simple, cost-effective, and ultrasensitive way by substituting the target-specific aptamer sequence. (C) 2015 Elsevier B.V. All rights reserved.
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