您好,欢迎访问中国水产科学研究院 机构知识库!

Morphology, sex steroid level and gene expression analysis in gonadal sex reversal of triploid female (XXX) rainbow trout (Oncorhynchus mykiss)

文献类型: 外文期刊

作者: Xu, Gefeng 1 ; Huang, Tianqing 1 ; Jin, Xian 2 ; Cui, Cunhe 2 ; Li, Depeng 2 ; Sun, Cong 2 ; Han, Ying 1 ; Mu, Zhenbo 3 ;

作者机构: 1.Northeast Agr Univ, Dept Anim Sci & Technol, Harbin, Heilongjiang, Peoples R China

2.Harbin Acad Agr Sci, Harbin, Peoples R China

3.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin, Peoples R China

关键词: Rainbow trout;Sex reversal;Triploid;Sex steroid;Gene expression

期刊名称:FISH PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:2.794; 五年影响因子:2.876 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In non-mammalian vertebrates, estrogens and expressions of cyp19a1 and foxl2 play critical roles in maintaining ovary differentiation and development, while dmrt1 and sox9 are male-specific genes in testicular differentiation and are highly conserved. In order to deeply understand the morphological change, sex steroids level and molecular mechanism of triploid female gonadal reversal in rainbow trout, we studied the ovary morphology, tendency of estradiol-17 beta (E2) and testosterone (T) levels and the relative expressions of dmrt1, cyp19a1, sox9 and foxl2 in juvenile and adult fish. Our results demonstrated that the development of triploid female gonads in rainbow trout went through arrested development, oocytes dedifferentiation, ovary reconstruction and sex reversal finally. During early gonadal development (154-334 days post-fertilization), the expressions of foxl2 and cyp19a1 increased linearly, while expressions of dmrt1 and sox9 were extremely suppressed, and E2 level was higher, while T level was lower. During the mid-to-late period of triploid female gonadal development (574-964 days post-fertilization), the expressions of dmrt1 and sox9 remained high and were very close to the quantity of diploid male genes, and T levels were even reaching diploid male plasma concentrations, while expressions of cyp19a1 and foxl2 were decreased, leading to decrease in E2 level. We realized that the development model of rainbow trout triploid female gonads was extremely rare, and the regulatory mechanism was very special. Genes involved in gonadal development and endogenous estrogens are pivotal factors in fish natural sex reversal.

  • 相关文献

[1]Effect of follicle cell autophagy on gonadal development of triploid female rainbow trout (Oncorhynchus mykiss). Huang, Tianqing,Sun, Huizhi,Wang, Yanna,Wang, Xingran,Han, Ying,Xu, Gefeng. 2018

[2]Expression of Proliferation Cell Nuclear Antigen (PCNA) in Peripheral Erythrocytes of Triploid Rainbow Trout Oncorhynchus mykiss (Walbaum). Liu, Yi,Fan, Zhaoting,Liu, Haijin,Wang, Bingqian. 2012

[3]Gender and gonadal maturity stage identification of captive Chinese sturgeon, Acipenser sinensis, using ultrasound imagery and sex steroids. Du, Hao,Leng, Xiaoqian,Zhang, Shuhuan,Luo, Jiang,Liu, Zhigang,Qiao, Xingmei,Wei, Qiwei,Zhang, Xiaoyan,Kynard, Boyd.

[4]Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis). Dong Xiaoli,Chen Songlin,Shao Changwei,Dong Xiaoli,Ji Xiangshan. 2011

[5]Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17 beta-estradiol during early life stages. Hu Peng,Hu Peng,Liu Bin,Meng Zhen,Liu Xinfu,Jia Yudong,Lei Jilin,Yang Zhi. 2017

[6]Characterization of vasa in the gonads of different ploidy fish. Yu, Fan,Zhong, Huan,Liu, Gang,Liu, Shaojun,Zhang, Zhuohui,Zhou, Yi,Tao, Min,Liu, Yun,Yu, Fan.

[7]Complete mitochondrial DNA genome of triploid pengze crucian carp (Carassius auratus). Li, Zhong,Liang, Hong-wei,Zou, Gui-wei. 2016

[8]Preliminary study of an oral vaccine against infectious hematopoietic necrosis virus using improved yeast surface display technology. Zhao, Jing-Zhuang,Xu, Li-Ming,Liu, Miao,Cao, Yong-Sheng,Yin, Jia-Sheng,Liu, Hong-Bai,Lu, Tong-Yan,LaPatra, Scott E..

[9]Estimation of genetic parameters for growth traits in a breeding program for rainbow trout (Oncorhynchus mykiss) in China. Hu, G.,Gu, W.,Bai, Q. L.,Wang, B. Q.. 2013

[10]Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model. Cao, Yongsheng,Xu, Liming,Li, Shaowu,Wang, Di,Zhao, Jingzhuang,Liu, Hongbai,Lu, Tongyan,Cao, Yongsheng,Zhang, Qiya,Feng, Jian. 2017

[11]Cadmium, chromium, and copper induce polychromatocyte micronuclei in carp (Cyprinus carpio L.). Zhu, Y,Wang, J,Bai, Y,Zhang, R.

[12]Two transcripts of HMG-CoA reductase related with developmental regulation from Scylla paramamosain: Evidences from cDNA cloning and expression analysis. Zhao, Ming,Jiang, Keji,Song, Wei,Ma, Chunyan,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Qiao, Zhenguo,Zhang, Fengying,Ma, Lingbo,Zhao, Ming,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Chen, Kai.

[13]Transcriptome analysis and discovery of genes involved in immune pathways in large yellow croaker (Larimichthys crocea) under high stocking density stress. Sun, Peng,Bao, Peibo,Tang, Baojun,Bao, Peibo.

[14]Growth performance and TOR pathway gene expression of juvenile blunt snout bream, Megalobrama amblycephala, fed with diets replacing fish meal with cottonseed meal. Zhou, Qun-Lan,Habte-Tsion, Habte-Michael,Ge, Xianping,Zhou, Qun-Lan,Ge, Xianping,Liu, Bo,Xie, Jun,Ren, Mingchun,Miao, Linghong,Pan, Liangkun.

[15]Dietary lipid levels impact lipoprotein lipase, hormone-sensitive lipase, and fatty acid synthetase gene expression in three tissues of adult GIFT strain of Nile tilapia, Oreochromis niloticus. Tian, Juan,Wu, Fan,Yang, Chang-Geng,Jiang, Ming,Liu, Wei,Wen, Hua,Tian, Juan,Jiang, Ming.

[16]Molecular characteristics of the HSP70 gene and its differential expression in female and male golden apple snails (Pomacea canaliculata) under temperature stimulation. Song, Hong-Mei,Mu, Xi-Dong,Gu, Dang-En,Luo, Du,Yang, Ye-Xin,Xu, Meng,Luo, Jian-Ren,Hu, Yin-Chang,Zhang, Jia-En.

[17]Phenoscape: Identifying Candidate Genes for Evolutionary Phenotypes. Edmunds, Richard C.,Westerfield, Monte,Su, Baofeng,Dunham, Rex A.,Balhoff, James P.,Dahdul, Wasila M.,Lapp, Hilmar,Vision, Todd J.,Eames, B. Frank,Dahdul, Wasila M.,Mabee, Paula M.,Lundberg, John G.,Vision, Todd J.,Edmunds, Richard C.,Su, Baofeng,Balhoff, James P.,Lapp, Hilmar.

[18]A manganese superoxide dismutase (MnSOD) from ark shell, Scapharca broughtonii: Molecular characterization, expression and immune activity analysis. Zheng, Libing,Wu, Biao,Liu, Zhihong,Tian, Jiteng,Zhou, Liqing,Sun, Xiujun,Yang, Aiguo,Zheng, Libing,Yu, Tao.

[19]beta-Defensin in Nile tilapia (Oreochromis niloticus): Sequence, tissue expression, and anti-bacterial activity of synthetic peptides. Dong, Jun-Jian,Wu, Fang,Ye, Xing,Sun, Cheng-Fei,Tian, Yuan-Yuan,Lu, Mai-Xin,Zhang, Rui,Chen, Zhi-Hang,Wu, Fang,Ye, Xing,Tian, Yuan-Yuan,Zhang, Rui,Chen, Zhi-Hang.

[20]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

作者其他论文 更多>>