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Promoter regulatory domain identification of cassava starch synthase IIb gene in transgenic tobacco

文献类型： 外文期刊

作者： Guan, Zhihui 1 ; Chen, Xin 1 ; Xie, Hairong 1 ; Wang, Wenquan 1 ;

作者机构： 1.CATAS, ITBB, Haikou 571101, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

关键词： Soluble starch synthase;Promoter;Cis-element;Genetic transformation;GUS protein

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

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收录情况： SCI

摘要： Soluble starch synthase is a key enzyme in the starch biosynthesis pathway, and its enzyme activity significantly influences starch components in cassava storage root. However, studies on the regulation mechanism of soluble starch synthase gene are rare. In this study, we cloned the 5' flanking sequence of the MeSSIIb gene and predicted the distribution of cis-elements. The region from -453 to -1 was considered the primary core promoter by the quantitative detection of GUS activity in transgenic tobacco plants containing 5' truncated promoters fused with the GUS gene. Analysis results clarified that the region from -531 to -454 significantly repressed promoter activity. The region from -453 to -388 was a repressive domain of ethylene, and some unknown drought responsive cis-elements were located in the region from -387 to -1. These findings will provide useful information on the functional assay and transcriptional regulation mechanisms of the MeSSIIb gene. (C) 2016 Elsevier Masson SAS. All rights reserved.

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