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Knockdown of a putative insulin-like peptide gene LdILP2 in Leptinotarsa decemlineata by RNA interference impairs pupation and adult emergence

文献类型： 外文期刊

作者： Fu, Kai-Yun 1 ; Zhu, Tao-Tao 1 ; Guo, Wen-Chao 2 ; Ahmat, Tursun 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

关键词： Leptinotarsa decemlineata;Insulin-like peptide;Juvenile hormone;20-Hydroxyecdysone;Metamorphosis

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： Five insulin-like peptide LdILP genes were identified in Leptinotarsa decemlineata. All of them contained three exons and two introns, with three genes tandemly arrayed and well separated from the other two. Phylogenetic analysis revealed that the three LdILPs from three tandemly-arrayed genes grouped with TcILP1, whereas the other two resembled with TcILP2 and TcILP4 from Tribolium castaneum. Thus, the five LdILP genes were provisionally named LdILP1a, LdILP1 b, LdILP1c, LdILP2 and LdILP4. LdILP2 was widely expressed in several tissues such as the brain-corpora cardiaca-corpora allata (BR-CC-CA) complex, gut and fat body. In contrast, LdILP1 a and LdILP1b were only transcribed in BR-CC-CA, LdILP4 was in ovaries, and LdILP1c was in both BR-CC-CA and ovaries. Ingestion of double-stranded RNAs (dsRNAs) targeting LdILP2 (dsLdILP2-1 and dsLdILP2-2) specifically knocked down LdILP2 and upregulated the transcription of both LdInR and Ld4EBP, indicating insulin/insulin-like growth factor signaling pathway (IIS) was inhibited. Approximately 50% of the LdILP2 RNAi larvae did not normally pupate and about 50% of the LdILP2 RNAi pupae did not emerge. Moreover, silencing LdILP2 reduced the expression of a juvenile hormone (JH) biosynthesis gene, lowered JH titer and disturbed JH signaling. Finally, knocking down LdILP2 inhibited an ecdysteroidogenesis gene, decreased 20-hydroxyecdysone (20E) titer, and repressed the expression of two 20E-response genes LdHR3 and LdFIZ-F1. Thus, the IIS pathway is involved in larval-pupal metamorphosis by modification of both JH and 20E signaling in L decemlineata. (C) 2016 Elsevier B.V. All rights reserved.

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