Selective electrochemical determination of glutathione from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis
文献类型： 外文期刊
作者： Zhao, Lingzhi 1 ; Zhao, Liu 2 ; Miao, Yanqing 1 ; Zhang, Chengxiao 3 ;
作者机构： 1.Xian Med Coll, Dept Pharm, Xian 710021, Peoples R China
2.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
3.Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
关键词： Glutathione (GSH);Biothiols;Electrochemical detection;Catechol derivatives;Single-walled carbon nanotubes (SWNTs)
期刊名称：ELECTROCHIMICA ACTA （ 影响因子：6.901； 五年影响因子：6.016 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： A highly selective method for the electrochemical detection of reduced glutathione (GSH) has been developed on the basis of the product of a reaction between GSH and catechol as the electron mediator on single-walled carbon nanotubes modified glassy carbon electrode. A polymerization-adduct ECE mechanism at SWNTs/GCE was proposed. The oxidation peak potential was -0.160 V (vs. Ag/AgCl, sat. KCl) and the oxidation peak current showed a dependence relationship with GSH concentration in the range of 1.0 similar to 500.0 mu M with a detection limit of 0.5 mu M GSH. The developed method can discriminate GSH from other thiol species such as L-cysteine (Cys) and cysteamine hydrochloride (Cym) within their certain concentration ranges. The as-established electrochemical method has been successfully applied for the determination of GSH from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis. (C) 2016 Elsevier Ltd. All rights reserved.
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