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Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed

文献类型: 外文期刊

作者: Guo, Xia 1 ; Chen, Dan-Dan 1 ; Peng, Kai-Song 1 ; Cui, Zheng-Wei 1 ; Zhang, Xu-Jie 1 ; Li, Shun 1 ; Zhang, Yong-An 1 ;

作者机构: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

3.Anhui Agr Univ, Hefei 230036, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

关键词: Bacillus subtilis;Probiotic;Stress condition resistance;Anti-inflammation;Grass carp

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Bacillus subtilis is widely used as probiotic species in aquaculture for water quality control, growth promoting, or immunity enhancing. The aim of this study is to find novel B. subtilis strains from fish as potential probiotics for aquaculture. Eleven B. subtilis isolates derived from the intestinal tract of grass carp were identified by gene sequencing and biochemical tests. These isolates were classified into 4 groups, and the representatives (GC-5, GC-6, GC-21 and GC-22) of each group were further investigated for antibiotic susceptibility, sporulation rate, biofilm formation, activity against pathogenic bacteria, resistance to stress conditions of intestinal tract (high percentage of bile and low pH) and high temperature, which are important for probiotics to be used as feed additives. Additionally, the adhesion properties of the 4 characterized strains were assessed using Caco-2 cell and gut mucus models. The results showed that the 4 strains differed in their capacities to adhere to intestinal epithelial cells and mucus. Furthermore, the strains GC-21 and GC-22 up-regulated the expression levels of IL-10 and TGF-beta but down-regulated IL-1 beta, suggesting their potential anti-inflammatory abilities. Based on physiological properties of the 4 characterized B. subtilis strains, one or more strains may have potential to be used as probiotics in aquaculture. (C) 2016 Elsevier Ltd. All rights reserved.

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