An enzyme-free catalytic DNA circuit for amplified detection of aflatoxin B1 using gold nanoparticles as colorimetric indicators
文献类型： 外文期刊
作者： Chen, Junhua 1 ; Wen, Junlin 1 ; Zhuang, Li 1 ; Zhou, Shungui 1 ;
作者机构： 1.Guangdong Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Agr Environm Pollut Integrated, Guangzhou 510650, Guangdong, Peoples R China
期刊名称：NANOSCALE （ 影响因子：7.79； 五年影响因子：7.632 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： An enzyme-free biosensor for the amplified detection of aflatoxin B1 has been constructed based on a catalytic DNA circuit. Three biotinylated hairpin DNA probes (H1, H2, and H3) were designed as the assembly components to construct the sensing system (triplex H1-H2-H3 product). Cascaded signal amplification capability was obtained through toehold-mediated strand displacement reactions to open the hairpins and recycle the trigger DNA. By the use of streptavidin-functionalized gold nanoparticles as the signal indicators, the colorimetric readout can be observed by the naked eye. In the presence of a target, the individual nanoparticles (red) aggregate into a cross-linked network of nanoparticles (blue) via biotin-streptavidin coupling. The colorimetric assay is ultrasensitive, enabling the visual detection of trace levels of aflatoxin B1 (AFB1) as low as 10 pM without instrumentation. The calculated limit of detection (LOD) is 2 pM in terms of 3 times standard deviation over the blank response. The sensor is robust and works even when challenged with complex sample matrices such as rice samples. Our sensing platform is simple and convenient in operation, requiring only the mixing of several solutions at room temperature to achieve visible and intuitive results, and holds great promise for the point-of-use monitoring of AFB1 in environmental and food samples.
- 相关文献
作者其他论文 更多>>
-
Heavy Metal-Induced Assembly of DNA Network Biosensor from Double-Loop Hairpin Probes for Ultrasensitive Detection of UO2+2 ° in Water and Soil Samples
作者：Wang, Xu;Guo, Junhui;Chen, Junhua;Lv, Yiwen;Chen, Manjia;Tong, Hui;Chen, Junhua;Liu, Chengshuai
关键词：
-
Intelligent monitoring of the available lead (Pb) and cadmium (Cd) in soil samples based on half adder and half subtractor molecular logic gates
作者：Chen, Junhua;Liu, Chengshuai;Chen, Junhua;Lv, Yiwen;Chen, Manjia;Tong, Hui;Wang, Xu
关键词：Molecular logic gate; Intelligent sensing; DNA recognition; Heavy metal; Available Pb; Available Cd
-
Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures
作者：Tang, Jia;Zhuang, Li;Ma, Jinlian;Tang, Ziyang;Yu, Zhen;Zhou, Shungui
关键词：
-
Effect of ferrihydrite biomineralization on methanogenesis in an anaerobic incubation from paddy soil
作者：Zhuang, Li;Xu, Jielong;Tang, Jia;Zhou, Shungui
关键词：ferrihydrite mineralization;methanogenesis;magnetite;direct electric syntrophy;Geobacter
-
Facile Synthesis of MnO2/Polypyrrole/MnO2 Multiwalled Nanotubes as Advanced Electrocatalysts for the Oxygen Reduction Reaction
作者：Yuan, Haoran;Deng, Lifang;Chen, Yong;Tang, Jiahuan;Zhou, Shungui;Yuan, Yong;Yuan, Haoran;Deng, Lifang;Chen, Yong
关键词：hydrothermal synthesis;microbial fuel cells;MnO2/Polypyrrole/MnO2;nanotubes;oxygen reduction reaction
-
Genome sequence of a dissimilatory Fe(III)-reducing bacterium Geobacter soli type strain GSS01(T)
作者：Yang, Guiqin;Yang, Guiqin;Chen, Shanshan;Zhou, Shungui;Yang, Guiqin;Liu, Yongfeng
关键词：Geobacter soli;Extracellular electron transfer;Insoluble Fe(III) oxides reduction;Cytochrome;Pilin protein
-
Axial Ligation of Heme in c-Type Cytochromes of Living Shewanella oneidensis: A New Insight into Enhanced Extracellular Electron Transfer
作者：Yuan, Yong;Zhou, Shungui;Li, Laicai
关键词：axial ligation;c-type cytochromes;density functional calculations;extracellular electron transfer;Shewanella oneidensis
