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IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY)

文献类型： 外文期刊

作者： Shi, Ji-Feng 1 ; Mu, Li-Li 1 ; Guo, Wen-Chao 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Educ Minist, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi, Peoples R China

关键词： Leptinotarsa decemlineata;chitin synthase;20-hydroxyecdysone;juvenile hormone

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Chitin synthase (ChS) plays a critical role in chitin synthesis and excretion. In this study, two ChS genes (LdChSA and LdChSB) were identified in Leptinotarsa decemlineata. LdChSA contains two splicing variants, LdChSAa and LdChSAb. Within the first, second, and third larval instars, the mRNA levels of LdChSAa, LdChSAb, and LdChSB coincide with the peaks of circulating 20-hydroxyecdysone (20E) and juvenile hormone (JH). In vitro culture of midguts and an in vivo bioassay revealed that 20E and an ecdysteroid agonist halofenozide stimulated the expression of the three LdChSs. Conversely, a reduction of 20E by RNA interference (RNAi) of an ecdysteroidogenesis gene LdSHD repressed the expression of these LdChSs, and ingestion of halofenozide by LdSHD RNAi larvae rescued the repression. Moreover, disruption of 20E signaling by RNAi of LdEcR, LdE75, LdHR3, and LdFTZ-F1 reduced the expression levels of these genes. Similarly, in vitro culture and an in vivo bioassay showed that exogenous JH and a JH analog methoprene activated the expression of the three LdChSs, whereas a decrease in JH by RNAi of a JH biosynthesis gene LdJHAMT downregulated these LdChSs. It seems that JH upregulates LdChSs at the early stage of each instar, whereas a 20E pulse triggers the transcription of LdChSs during molting in L. decemlineata.

  • 相关文献

[1]RNA interference suppression of the receptor tyrosine kinase Torso gene impaired pupation and adult emergence in Leptinotarsa decemlineata. Zhu, Tao-Tao,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[2]Transcription response of three putative trehalase genes to hormonal stimulation in the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Shi, Ji-Feng,Sun, Qiang-Kun,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[3]Involvement of FTZ-F1 in the regulation of pupation in Leptinotarsa decemlineata (Say). Liu, Xin-Ping,Fu, Kai-Yun,Lu, Feng-Gong,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[4]Nuclear receptor ecdysone-induced protein 75 is required for larval-pupal metamorphosis in the Colorado potato beetle Leptinotarsa decemlineata (Say). Guo, W-C.,Liu, X-P.,Fu, K-Y.,Shi, J-F.,Lu, F-G.,Li, G-Q.,Guo, W-C..

[5]Knockdown of a putative insulin-like peptide gene LdILP2 in Leptinotarsa decemlineata by RNA interference impairs pupation and adult emergence. Fu, Kai-Yun,Zhu, Tao-Tao,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun.

[6]Transcription changes of a putative trehalose-6-phosphate synthase gene in response to hormone stimulation in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Xu, Qing-Yu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[7]RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Mu, Li-Li,Chen, Xu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[8]Identification of ten mevalonate enzyme-encoding genes and their expression in response to juvenile hormone levels in Leptinotarsa decemlineata (Say). Li, Qian,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[9]Leptinotarsa cap 'n' collar isoform C/Kelch-like ECH associated protein 1 signaling is critical for the regulation of ecdysteroidogenesis in the larvae. Sun, Qiang-Kun,Meng, Qing-Wei,Xu, Qing-Yu,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao.

[10]Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference. Guo, Wen-Chao,Liu, Xin-Ping,Fu, Kai-Yun,Shi, Ji-Feng,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[11]Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Fu, Kai-Yun,Li, Qian,Zhou, Li-Tao,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[12]A putative Delta(1)-pyrroline-5-carboxylate synthetase involved in the biosynthesis of proline and arginine in Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[13]Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae. Guo, Wen-Chao,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao,Yang, Shuai,Li, Xiao-Xu.

[14]Chlorantraniliprole susceptibility in leptinotarsa decemlineata in the north Xinjiang Uygur autonomous region in China. Jiang, Wei-Hua,Lu, Wei-Ping,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun.

[15]Physiological roles of trehalose in Leptinotarsa larvae revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes. Shi, Ji-Feng,Xu, Qing-Yu,Sun, Qiang-Kun,Meng, Qing-Wei,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[16]CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[17]Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle. Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao.

[18]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

[19]Insecticide resistance status of Colorado potato beetle (Coleoptera: Chrysomelidae) adults in Northern Xinjiang Uygur autonomous region. Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Guo, Wen-Chao.

[20]The subunit gene Ld alpha 1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata. Qu, Yang,Chen, Jinhua,Li, Chenge,Wang, Qiang,Han, Zhaojun,Jiang, Weihua,Guo, Wenchao.

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