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Antiviral activity of recombinant porcine surfactant protein A against porcine reproductive and respiratory syndrome virus in vitro

文献类型： 外文期刊

作者： Li, Lan 1 ; Zheng, Qisheng 2 ; Zhang, Yuanpeng 2 ; Li, Pengcheng 2 ; Fu, Yanfeng 3 ; Hou, Jibo 2 ; Xiao, Xilong 1 ;

作者机构： 1.China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has caused significant economic losses in the swine industry worldwide. However, there is not an ideal vaccine to provide complete protection against PRRSV. Thus, the need for new antiviral strategies to control PRRSV still remains. Surfactant protein A (SP-A) belongs to the family of C-type lectins, which can exert antiviral activities. In this present study, we assessed the antiviral properties of recombinant porcine SP-A (RpSP-A) on PRRSV infection in Marc 145 cells and revealed its antiviral mechanism using a plaque assay, real-time qPCR, western blotting analysis and an attachment and penetration assay. Our results showed that RpSP-A could inhibit the infectivity of PRRSV in Marc 145 cells and could reduce the total RNA and protein level. The attachment assay indicated that RpSP-A in the presence of Ca2+ could largely inhibit Marc 145 cell attachment; however, in the penetration assay, it was relatively inactive. Furthermore, our study suggested that virus progeny released from infected Marc145 cells were blocked by RpSP-A from infecting other cells. We conclude that RpSP-A has antiviral activity against PRRSV, most probably by blocking viral attachment and the cell-to-cell transmission pathway, and therefore, RpSP-A holds promise as a novel antiviral agent against PRRSV.

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