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Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei

文献类型： 外文期刊

作者： Fu, Bo 1 ; Liu, Di 1 ; Ma, Hong 1 ; Guo, Zhen-Hua 1 ; Wang, Liang 1 ; Li, Zhong-Qiu 1 ; Peng, Fu-Gang 1 ; Bai, Jing 3 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Anim Husb Res, Harbin 150086, Peoples R China

2.Northeast Agr Univ, Coll Anim Sci, Harbin 150030, Peoples R China

3.Heilongjiang Univ Chinese Med, Modern Educ Technol & Informat Ctr, Harbin 150040, Peoples R China

关键词： cloning efficiency;nuclear factors;porcine oocytes;reprogram;somatic cell nuclear transfer;tetraploid

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

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收录情况： SCI

摘要： Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.

  • 相关文献

[1]Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities. Fu, Bo,Liu, Di,Ma, Hong,Zhang, Dong-Jie,Guo, Zhen-Hua,Fu, Bo,Ma, Jian-Zhang,An, Tie-Zhu,Ren, Liang,Liu, Di,Yang, Xiu-Qin,Guo, Yun-Yun,Zhu, Meng,Bai, Jing.

[2]Supplementation with glucose in PZM-3 medium improve the in vitro development of porcine transgenic cloned embryos. Fu, Bo,Liu, Di,Ma, Jian-zhang,An, Tie-zhu,Ren, Liang,Guo, Yun-yun,Kang, Ya,Zhao, Cheng-yue,Ma, Hong,Li, Zhong-qiu,Wang, Wen-tao,Bai, Jing. 2011

[3]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[4]Effect of Cycloheximide on the Development of Parthenogenetic Activation and Cloned Porcine Embryos. Ma, Hong,Fu, Bo,Li, Zhongqiu,Liu, Di,Zhao, Jinfeng,Fang, Qingchang,Ren, Liang. 2011

[5]Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos. Liu Di,Ma Hong,Wang Liang,Guo Zhen-hua,Li Zhong-qiu,Fu Bo,Liu Di,Fang Qing-chang,Ren Liang.

[6]Using a nano-flare probe to detect RNA in live donor cells prior to somatic cell nuclear transfer. Fu, Bo,Ma, Jian-zhang,An, Tie-zhu,Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-hua,Ren, Liang,Liu, Di,Yang, Xiu-qin,Zhu, Meng,Bai, Jing.

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