您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The role of Ca2+ mediated signaling pathways on the effect of taurine against Streptococcus uberis infection

文献类型： 外文期刊

作者： Dai, Bin 1 ; Zhang, Jinqiu 2 ; Liu, Ming 1 ; Lu, Jinye 3 ; Zhang, Yuanshu 1 ; Xu, Yuanyuan 1 ; Miao, Jinfeng 1 ; Yin, Yul 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Vaccine Engn & Technol China, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Agrianim Husb Vocat Coll, Taizhou 225300, Peoples R China

4.Chinese Acad Sci, Res Ctr Hlth Breeding Livestock & Poultry,Inst Su, Hunan Engn & Res Ctr Anim & Poultry Sci,Minist Ag, Key Lab Agroecol Subtrop Reg,Sci Observing & Expt, Changsha 410125, Hunan, Peoples R China

关键词： Streptococcus uberis;Taurine;Ca2+;Mouse mammary epithelial cell

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To provide insight into the mechanisms of taurine attenuation of pro-inflammatory response in mouse mammary epithelial cell line (EpH(4)-Ev, purchased by ATCC, USA) after Streptococcus uberis (S. uberis, 0140J) challenge, we infected MECs with S. uberis (2.5 x 10(7) cfu mL(-1), MOI = 10) for 3 h and quantified changes in TLR-2 and calcium (Ca2+) mediated signaling pathways. The results indicate that S. uberis infection significantly increases the expression of TLR-2, intracellular Ca2+ levels, PLC-gamma 1 and PKC-alpha, the activities of transcription factors NF-kappa B and NFAT, and related cytokines (TNF-alpha, IL-beta, IL-6, G-CSF, IL-2, KC, IL-15, FasL, MCP-1, and LIX) in culture supernatants. Taurine administration downregulated all these indices, the activities of NF-kappa B and NFAT. Cytokine secretions were similar using special PKC inhibitor Go 6983 and NFAT inhibitor VIVIT. Our data indicate that S. uberis infection induces pro-inflammatory response of MECs through a TLR-2 mediated signaling pathway. In addition, taurine can prevent MEC damage by affecting both PLC-gamma 1-Ca2+-PKC-alpha-NF-kappa B and PLC-gamma 1-Ca2+-NFATs signaling pathways. This is the first report to demonstrate the mechanisms of taurine attenuated pro-inflammatory response in MECs after S. uberis challenge. (C) 2016 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Taurine attenuates Streptococcus uberis-induced mastitis in rats by increasing T regulatory cells. Miao, Jinfeng,Zheng, Liuhai,Yu, Xiaoming,Zhu, Wei,Zou, Sixiang,Zhang, Jinqiu.

[2]The effect of taurine on the toll-like receptors/nuclear factor kappa B (TLRs/NF-kappa B) signaling pathway in Streptococcus uberis-induced mastitis in rats. Miao, Jinfeng,Zheng, Liuhai,Ma, Zili,Zhu, Wei,Zou, Sixiang,Zhang, Jinqiu. 2011

[3]The role of NADPH oxidase in taurine attenuation of Streptococcus uberis-induced mastitis in rats. Miao, Jinfeng,Ma, Zili,Zheng, Liuhai,Zhang, Jinqiu. 2013

[4]Promotion of photosynthesis in transgenic rice over-expressing of maize C-4 phosphoenolpyruvate carboxylase gene by nitric oxide donors. Chen Pingbo,Li Xia,Huo Kai,Wei Xiaodong,Lv Chuangen,Dai Chuanchao,Chen Pingbo,Huo Kai.

[5]Enhanced drought tolerance in transgenic rice over-expressing of maize C-4 phosphoenolpyruvate carboxylase gene via NO and Ca2+. Qian, Baoyun,Li, Xia,Liu, Xiaolong,Chen, Pingbo,Ren, Chengang,Qian, Baoyun,Liu, Xiaolong,Dai, Chuanchao.

[6]Ca~(2+)、CaM及CaM拮抗剂对粟酒裂殖酵母质膜H~+-ATP酶活性的影响. 林玲,袁生,陆玲,幸定坤,戴传超. 2006

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台