您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Stepwise modular pathway engineering of Escherichia coli for efficient one-step production of (2S)-pinocembrin

文献类型： 外文期刊

作者： Wu, Junjun 1 ; Zhang, Xia 1 ; Dong, Mingsheng 1 ; Zhou, Jingwen 2 ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210095, Jiangsu, Peoples R China

关键词： Plant natural product;Phenylpropanoids;Metabolic engineering;mRNA structure

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Developing efficient microbial processes to produce flavonoids has been a metabolic engineering goal over the past decade due to their important functions. Previously, the de novo production of the main flavonoid precursor (2S)-pinocembrin was achieved. However, low productivity and two separate fermentation steps made it inappropriate for industrial scale low-cost production. Here, a stepwise modular engineering approach was introduced to systematically identify and eliminate metabolic pathway bottlenecks. The overall pathway was firstly divided into four modules and analysis then revealed that efficient conversion of L-phenylalanine to (2S)-pinocembrin is the major limiting factor. Therefore, the pathway from L-phenylalanine to (2S)-pinocembrin was re-cast into three modules to alleviate this bottleneck by modifying both the gene copy number and promoter strength. Furthermore, the expression of the rate limiting enzyme PAL was found to be correlated with 5' region of mRNA structure. The efficiency of the synthetic pathway was then improved by customizing the PAL expression level based on modification of the mRNA secondary structure. Fed-batch cultivation of engineered strains in a 3-L fermentor resulted in a final (2S)-pinocembrin production of 432.4 mg/L. The results presented here pave the way for the development of an economical and simple process for microbial production of flavonoids. (C) 2016 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Efficient de novo synthesis of resveratrol by metabolically engineered Escherichia coli. Wu, Junjun,Zhou, Peng,Zhang, Xia,Dong, Mingsheng,Wu, Junjun,Zhou, Peng,Zhang, Xia,Dong, Mingsheng.

[2]Improving metabolic efficiency of the reverse beta-oxidation cycle by balancing redox cofactor requirement. Wu, Junjun,Zhang, Xia,Zhou, Peng,Huang, Jiaying,Li, Wei,Zhou, Ziyu,Chen, Yue,Liu, Yinghao,Dong, Mingsheng,Xia, Xiudong.

[3]A systematic optimization of medium chain fatty acid biosynthesis via the reverse beta-oxidation cycle in Escherichia coli. Wu, Junjun,Zhang, Xia,Dong, Mingsheng,Xia, Xiudong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台