您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells

文献类型： 外文期刊

作者： Gao, Quanxin 1 ; Xiao, Yingping 2 ; Zhang, Chenjie 1 ; Min, Minghua 1 ; Peng, Shiming 1 ; Shi, Zhaohong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Toll-like receptor 2;Silvery pomfret;Molecular cloning;Expression modulation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Toll-like receptor 2 (TLR2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. In this study, the full-length cDNA of TLR2 in silvery pomfret (Pampus argenteus) was cloned by homology cloning and the rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of TLR2 was 2932 bp, containing an open reading frame (ORF) of 2469 bp encoding 822 amino acids. A multiple alignment analysis of the silvery pomfret TLR2 protein-coding sequence with other known TLR2 sequences from Oplegnathus fasciatus, Epinephelus coioides, Larimichthys crocea, Miichthys miiuy, Oreochromis niloticus, Paralichthys olivaceus, Trematomus bernacchii, Sparta aurata, and Chionodraco hamatus exhibited a high degree of homology of 78.83%, 75.91%, 74.21%, 74.94%, 71.95%, 72.57%, 73.68%, 75%, and 72.52 respectively, between these fish. Analysis of the TLR2 domain structures indicated that TLR2 from the silvery pomfret has the typical structural features of proteins that belong to the TLR family, including one transmembrane domain, eleven leucine-rich repeats (LRRs), and one Toll/IL-1 receptor homology domain (TIR). In vitro immunostimulation experiments revealed that Lactobacillus plantarum and Clostridium butyricum induce high levels of TLR2 mRNA and protein expression, but they induce only moderate levels of IL-8 and TNF-alpha production compared to Vibrio anguillarum. This suggests that TLR2 might play a vital role in the L. plantarum and C butyricum-mediated immune response. In contrast, V. anguillarum significantly increased the secretion of IL-8 and TNF-alpha and induced cell apoptosis and necrosis. Due to the lower expression of TLR2 and higher levels of IL-8 and TNF-alpha induced by V. anguillarum, we hypothesize that a V anguillarum infection is independent of the TLR2-induced production of pro-inflammatory cytokines. These results indicate that TLR2 may be involved in molecular interactions between the host and commensal bacteria, that exist in the silvery pomfret intestinal tract. (C) 2016 Published by Elsevier Ltd.

  • 相关文献

[1]Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice. Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi,Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台