Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells
文献类型： 外文期刊
作者： Gao, Quanxin 1 ; Xiao, Yingping 2 ; Zhang, Chenjie 1 ; Min, Minghua 1 ; Peng, Shiming 1 ; Shi, Zhaohong 1 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab Marine & Estuarine Fisheries, Shanghai 200090, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Toll-like receptor 2;Silvery pomfret;Molecular cloning;Expression modulation
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Toll-like receptor 2 (TLR2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. In this study, the full-length cDNA of TLR2 in silvery pomfret (Pampus argenteus) was cloned by homology cloning and the rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of TLR2 was 2932 bp, containing an open reading frame (ORF) of 2469 bp encoding 822 amino acids. A multiple alignment analysis of the silvery pomfret TLR2 protein-coding sequence with other known TLR2 sequences from Oplegnathus fasciatus, Epinephelus coioides, Larimichthys crocea, Miichthys miiuy, Oreochromis niloticus, Paralichthys olivaceus, Trematomus bernacchii, Sparta aurata, and Chionodraco hamatus exhibited a high degree of homology of 78.83%, 75.91%, 74.21%, 74.94%, 71.95%, 72.57%, 73.68%, 75%, and 72.52 respectively, between these fish. Analysis of the TLR2 domain structures indicated that TLR2 from the silvery pomfret has the typical structural features of proteins that belong to the TLR family, including one transmembrane domain, eleven leucine-rich repeats (LRRs), and one Toll/IL-1 receptor homology domain (TIR). In vitro immunostimulation experiments revealed that Lactobacillus plantarum and Clostridium butyricum induce high levels of TLR2 mRNA and protein expression, but they induce only moderate levels of IL-8 and TNF-alpha production compared to Vibrio anguillarum. This suggests that TLR2 might play a vital role in the L. plantarum and C butyricum-mediated immune response. In contrast, V. anguillarum significantly increased the secretion of IL-8 and TNF-alpha and induced cell apoptosis and necrosis. Due to the lower expression of TLR2 and higher levels of IL-8 and TNF-alpha induced by V. anguillarum, we hypothesize that a V anguillarum infection is independent of the TLR2-induced production of pro-inflammatory cytokines. These results indicate that TLR2 may be involved in molecular interactions between the host and commensal bacteria, that exist in the silvery pomfret intestinal tract. (C) 2016 Published by Elsevier Ltd.
- 相关文献
作者其他论文 更多>>
-
The Effect of Clostridium butyricum-Derived Lipoteichoic Acid on Lipopolysaccharide-Stimulated Porcine Intestinal Epithelial Cells
作者：Chen, Qu;Ma, Lingyan;Wen, Yang;Lyu, Wentao;Yu, Minjie;Yang, Hua;Xiao, Yingping
关键词：
Clostridium butyricum ; inflammatory factors; IPEC-J2; lipopolysaccharide; lipoteichoic acid -
Extracellular vesicles derived from Lactobacillus johnsonii promote gut barrier homeostasis by enhancing M2 macrophage polarization
作者：Tao, Shiyu;Fan, Jinping;Li, Jingjing;Wu, Zhifeng;Yao, Yong;Liu, Xiangdong;Wei, Hong;Wang, Zhenyu;Wu, Yujun;Xiao, Yingping
关键词：Diarrhea; Microbiome; Probiotics; Extracellular vesicles; Macrophage
-
Metabolomic profiles in serum uncover novel biomarkers in children with Williams-Beuren syndrome
作者：Bai, Guannan;Chen, Weijun;Ji, Chai;Shen, Jiyang;Li, Fangfang;Jiang, Xiaoling;Yang, Yang;Yang, Yang;Wen, Yang;Xiao, Yingping;Tan, Danny Junyi;Chen, Jinluan
关键词：Williams-Beuren syndrome; Untargeted metabolomics; Serotonin; Tryptophan; Retrograde endocannabinoid signaling; Bile acids
-
Intestinal fungi biogeography, succession and its association with diarrhea in pigs
作者：Ren, Ruochen;Ma, Lingyan;Xiao, Yingping;Chen, Qu;Wen, Yang;Lyu, Wentao;Zhang, Xiaojun;Lou, Fangfang;Li, Yang
关键词：Diarrhea; Fungi; Growth stage; Mycobiome; Pigs
-
Enhancement of gut barrier integrity by a Bacillus subtilis secreted metabolite through the GADD45A-Wnt/β-catenin pathway
作者：Liu, Shiqi;Cai, Peiran;You, Wenjing;Yang, Mingshun;Tu, Yuang;Zhou, Yanbing;Wang, Yizhen;Shan, Tizhong;Liu, Shiqi;Cai, Peiran;You, Wenjing;Yang, Mingshun;Tu, Yuang;Zhou, Yanbing;Wang, Yizhen;Shan, Tizhong;Liu, Shiqi;Cai, Peiran;You, Wenjing;Yang, Mingshun;Tu, Yuang;Zhou, Yanbing;Wang, Yizhen;Shan, Tizhong;Valencak, Teresa G.;Xiao, Yingping
关键词：2-hydroxy-4-methylpentanoic acid; GADD45A; intestinal barrier; metabolites; probiotics; Wnt/
beta -catenin signaling -
Integrated 16S rRNA and metagenomic sequencing reveals the distribution of key antibiotic resistance genes in duck gut microbiota
作者：Zeng, Hongbo;Yang, Hua;Ma, Lingyan;Lu, Lizhi;Zeng, Tao;Xiao, Yingping;Lyu, Wentao;Fu, Zixian
关键词：Antibiotic resistance gene; 16S rRNA gene sequencing; Metagenomic analysis; Gut microbiota; Duck
-
Integrated Metabolome and Microbiome Analysis Reveals the Regulatory Effects of Fermented Soybean Meal on the Gut Microbiota of Late Gestation
作者：Li, Yantao;Ren, Ying;Li, Yantao;Fu, Lele;Chen, Yushi;Yang, Hua;Wang, Cheng;Yang, Hua;Xiao, Yingping
关键词：gut microbiota; metabolite; fermentation; soybean meal; late gestation
