A simple and efficient in planta transformation method for pommelo (Citrus maxima) using Agrobacterium tumefaciens
文献类型： 外文期刊
作者： Zhang, Yong-yan 1 ; Zhang, Dong-min 1 ; Zhong, Yun 2 ; Chang, Xiao-jun 1 ; Hu, Min-lun 2 ; Cheng, Chun-zhen 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China
关键词： Agrobacterium-mediated transformation;Citrus maxima;Dark incubation;in planta transformation
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
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收录情况： SCI
摘要： A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integration confirmation and transgenic plant propagation. The highest transformation efficiency (20.41%) was obtained when using hygromycin as selection marker, followed by Basta (1937%) and Kanamycin (3.21%). Trans genic plants obtained using this method could be graft-propagated in 3 months post transformation, which is shorter than the tissue-culture-dependent transformation. The transformation protocol presented here has great potential to be used for citrus genetic breeding and improvement. (C) 2016 Elsevier B.V. All rights reserved.
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