您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

H9 subtype influenza vaccine in MDCK single-cell suspension culture with stable expression of TMPRSS2: Generation and efficacy evaluation

文献类型： 外文期刊

作者： Feng, Lei 1 ; Tang, Yinghua 1 ; Wu, Peipei 1 ; Chu, Xuan 1 ; Wang, Weifeng 1 ; Hou, Jibo 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： Avian influenza virus;Immunization;MDCK cells;Single cell suspension culture;TMPRSS2

期刊名称：ENGINEERING IN LIFE SCIENCES （ 影响因子：2.678； 五年影响因子：2.704 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Vaccination is the most effective way to protect chickens from avian influenza pan-demics. Mammalian cells, especially MDCK cells, are being investigated as a good alternative to the embryonated chicken eggs for avian influenza vaccine production. In this work, we developed a new MDCK cell line, which has two main features: single-cell suspension growth and stable expression of human TMPRSS2 protein (transmembrane protease serine S1 member 2) anchored on cells membrane, named MDCK-Sus-TMPRSS2. Reverse-transcription polymerase chain reaction, western blot analysis and indirect immunofluorescence assay were employed to detect the expression of TMPRSS2 in the MDCK-Sus-TMPRSS2 cells. Then, H9 subtype avian influenza virus, NJ02/01 strain, was adapted in MDCK-Sus-TMPRSS2 cells with serial blind passages and propagated in stirred bioreactor with 9.5 log2/25 mu L hemag-glutination titers for inactivated avian influenza vaccine production without addition of exogenous trypsin. The MDCK-Sus-TMPRSS2 cell-derived H9 subtype avian influenza vaccine could induce high titers of hemagglutiniton inhibition antibodies in specific pathogen-free chickens and protect chickens against heterologous H9 sub-type influenza virus challenge, showing lower virus shedding rate and sickness rate than in the egg-derived vaccines group. High titers of H9 subtype-specific antibodies above 6 log2 were maintained for 6 months in commercial chickens vaccinated by the MDCK-Sus-TMPRSS2 cells derived H9 subtype avian influenza vaccine and there were no negative effects on body weight increase in these chickens. Taken together, the results of this work revealed that MDCK-Sus-TMPRSS2 cell line could be a promising and safe substitute for embryonated chicken eggs as a host cell line for H9 subtype avian influenza virus propagation.

  • 相关文献

[1]Studies on enhancing embryo quantity and quality by immunization against inhibin in repeatedly superovulated Holstein heifers and the associated endocrine mechanisms. Liu, Y. P.,Chen, R. A.,Mao, X. B.,Wei, Y. M.,Yu, J. N.,Li, H.,Shi, Z. D..

[2]Immunization against inhibin improves in vivo and in vitro embryo production. Yan, Leyan,Li, Hui,Shi, Zhendan.

[3]Detection of Avian H7N9 Influenza A Viruses in the Yangtze Delta Region of China During Early H7N9 Outbreaks. Li, Yin,Huang, Xin-Mei,Zhao, Dong-Min,Liu, Yu-Zhuo,He, Kong-Wang,Xie, Xing-Xing,Han, Kai-Kai,Liu, Xiao-Yan,Yang, Jing,Li, Yin,Huang, Xin-Mei,Zhao, Dong-Min,Liu, Yu-Zhuo,He, Kong-Wang,Xie, Xing-Xing,Han, Kai-Kai,Liu, Xiao-Yan,Yang, Jing,Liu, Yao-Xing,Chen, Chang-Hai,Long, Li-Ping,Xu, Yifei,Wan, Xiu-Feng,Zhang, You-Fa,Fan, Feng,Webby, Richard.

[4]Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens. Tang, Yinghua,Lu, Jihu,Wu, Peipei,Liu, Zhenxing,Tian, Zhen,Zha, Guofei,Chen, Hui,Wang, Qiaochu,Hou, Fengxiang,Hou, Jibo,Tang, Yinghua,Wang, Qiaoxiu,Kang, Sang-Moo.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台