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Characterization of the Arabidopsis thaliana heme oxygenase 1 promoter in response to salinity, iron deficiency, and mercury exposure

文献类型: 外文期刊

作者: Wang, F. -Q. 1 ; Yang, J. 2 ; Dai, C. 1 ; Wu, M. -Z. 3 ; Zhang, Y. -H. 1 ; Shen, W. -B. 1 ;

作者机构: 1.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Peoples R China

3.China Natl Tobacco Corp, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China

关键词: beta-glucuronidase;MYB binding site;transgenic plants;5 '-untranslated region

期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )

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收录情况: SCI

摘要: The Arabidopsis heme oxygenase 1 (HY1) plays a significant role in the signal transduction of abiotic stimuli and hormonal response. To characterize the HY1 promoter, an approximately 1.8 kb of it (pHY1, -1666 to +132) and its deletion fragments (5D1, -1528 to +132; 5D2, -1109 to +132; 5D3, -688 to +132; 5D4, -169 to +132; 3D1, -1666 to +100; 3D2, -1666 to -1; and 3D3, -1666 to -170), were fused to the beta-glucuronidase (GUS) reporter gene and transformed into Arabidopsis. The transgenic plants were subjected to several environmental stimuli (especially to mild salinity, iron deficiency, and mercury exposure). The results show that the region from +1 to +100 in the 5'-untranslated region were essential for HY1 basal promoter activity. The induced GUS activities under NaCl and H2O2 treatments were slowed down by the progressive 5' deletion (from -1666 to -688) and correlated with the reduced numbers of myeloblastosis (MYB) binding sites (MBSs; -1542, -1333, -1078, and -177). The MBS-free promoter construct 5D4 (-169 to +132), however, fully lost the inducibility. Therefore, we propose that the MBS elements existing in the HY1 promoter might be crucial for salinity-induced HY1 up-regulation in an H2O2-dependent fashion. Moreover, the regions from -169 to -1 and -688 to -169 were presumed as the regulatory regions of HY1 promoter in response to iron deficiency and mercury exposure, respectively.

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