Molecular cloning, mRNA expression and characterization of membrane-bound hemoglobin in oriental river prawn Macrobrachium nipponense
文献类型: 外文期刊
作者: Sun, Shengming 1 ; Xuan, Fujun 2 ; Fu, Hongtuo 1 ; Zhu, Jian 1 ; Ge, Xianping 1 ; Wu, Xugan 3 ;
作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Minist Agr, Wuxi 214081, Peoples R China
2.Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng City 224002, Jiangsu, Peoples R China
3.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
关键词: Macro brachium nipponense;Membrane-bound hemoglobin;Hypoxia;Oxidative stress;Gill
期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY ( 影响因子:2.32; 五年影响因子:2.465 )
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收录情况: SCI
摘要: Most hemoglobins are respiratory proteins and are ubiquitous in animals, bacteria, fungi, protists, and plants. In this study, we describe a membrane-bound hemoglobin in the oriental river prawn Macrobrachium nipponense (MnHb), which also expresses hemocyanin. MnHb cDNA was cloned using the rapid amplification of cDNA ends (RACE) approach, which afforded a 1201 bp gene encoding a 193 amino acid polypeptide. Bioinformatic evaluation suggested MnHb is membrane anchored by N-myristoylation, and immunofluorescence confirmed its location in the membrane of chief cells in the gill. The effect of hypoxia on MnHb expression was investigated, and reverse transcription PCR (RT-PCR) and Western blotting showed that MnHb was expressed almost exclusively in the gill. Quantitative RT-PCR revealed a significant increase in expression after 6 h of hypoxia, and levels peaked at 24 h due to oxidative stress. Exposure of cultured prawns to the stress inducer H2O2 significantly up regulated the expression of MnHb in a dose-dependent manner. MnHb may have a role in protecting cell membrane lipids from damage by reactive oxygen species. (C) 2017 Elsevier Inc. All rights reserved.
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