您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning, mRNA expression and characterization of membrane-bound hemoglobin in oriental river prawn Macrobrachium nipponense

文献类型： 外文期刊

作者： Sun, Shengming 1 ; Xuan, Fujun 2 ; Fu, Hongtuo 1 ; Zhu, Jian 1 ; Ge, Xianping 1 ; Wu, Xugan 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Minist Agr, Wuxi 214081, Peoples R China

2.Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng City 224002, Jiangsu, Peoples R China

3.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

关键词： Macro brachium nipponense;Membrane-bound hemoglobin;Hypoxia;Oxidative stress;Gill

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY （ 影响因子：2.32； 五年影响因子：2.465 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Most hemoglobins are respiratory proteins and are ubiquitous in animals, bacteria, fungi, protists, and plants. In this study, we describe a membrane-bound hemoglobin in the oriental river prawn Macrobrachium nipponense (MnHb), which also expresses hemocyanin. MnHb cDNA was cloned using the rapid amplification of cDNA ends (RACE) approach, which afforded a 1201 bp gene encoding a 193 amino acid polypeptide. Bioinformatic evaluation suggested MnHb is membrane anchored by N-myristoylation, and immunofluorescence confirmed its location in the membrane of chief cells in the gill. The effect of hypoxia on MnHb expression was investigated, and reverse transcription PCR (RT-PCR) and Western blotting showed that MnHb was expressed almost exclusively in the gill. Quantitative RT-PCR revealed a significant increase in expression after 6 h of hypoxia, and levels peaked at 24 h due to oxidative stress. Exposure of cultured prawns to the stress inducer H2O2 significantly up regulated the expression of MnHb in a dose-dependent manner. MnHb may have a role in protecting cell membrane lipids from damage by reactive oxygen species. (C) 2017 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Transcriptomic response to water accommodated fraction of crude oil exposure in the gill of Japanese flounder, Paralichthys olivaceus. Zhu, Lin,Qu, Keming,Xia, Bin,Sun, Xuemei,Chen, Bijuan,Xia, Bin.

[2]Variation of antioxidant/detoxification enzyme activities in response to benzo[a]pyrene in the gazami crab Portunus trituberculatus. Li, Lei,Jiang, Mei,Shen, Xinqiang.

[3]Effects of water-borne copper on isozymes of gill in Eriocheir sinensis. Zhao, Yun-long,Yang, Zhi-biao,Hua, Xiu-guo,Li, Na,Fan, Yu-jie,Yang, Zhi-biao,Zhang, Wen,Zhu, Jian-guo,Cui, Li,Yang, Jian. 2010

[4]Cloning and expression analysis of a heat shock protein 90 beta isoform gene from the gills of Wuchang bream (Megalobrama amblycephala Yih) subjected to nitrite stress. Sun, S. M.,Zhu, J.,Ge, X. P.,Zhang, C. F.,Miao, L. H.,Jiang, X. J.. 2015

[5]Diversification of the duplicated Rab1a genes in a hypoxia-tolerant fish, common carp (Cyprinus carpio). Zhao, Zi-Xia,Xu, Jian,Xu, Ru,Li, Jiong-Tang,Zhang, Yan,Xu, Peng,Sun, Xiao-Wen,Cao, Ding-Chen,Sun, Xiao-Wen.

[6]Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Gu, Zhimin,Xuan, Fujun.

[7]Molecular characterization and mRNA expression of hypoxia inducible factor-1 and cognate inhibiting factor in Macrobrachium nipponense in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Wenyi,Xuan, Fujun.

[8]Comparative proteomic study of the response to hypoxia in the muscle of oriental river prawn (Macrobrachium nipponense). Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Yiwen,Xuan, Fujun.

[9]Respiratory response of grass carp Ctenopharyngodon idellus to dissolved oxygen changes at three acclimation temperatures. Zhao, Zhigang,Xu, Qiyou,Dong, Shuanglin. 2018

[10]Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun. 2017

[11]Editorial: Eutrophication and hypoxia and their impacts on the ecosystem of the Changjiang Estuary and adjacent coastal environment. Zhang, Jing,Xiao, Tian,Huang, Daji,Liu, Su Mei,Fang, Jianguang. 2016

[12]Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun,Gu, Zhimin. 2015

[13]Duplication and differentiation of common carp (Cyprinus carpio) myoglobin genes revealed by BAC analysis. Zhao, Zi-Xia,Xu, Peng,Deng, Hai-Xia,Zhang, Yan,Xu, Li-Ming,Li, Jiong-Tang,Xu, Jian,Sun, Xiao-Wen,Cao, Ding-Chen,Kuang, You-Yi,Sun, Xiao-Wen.

[14]Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia. Sun, Shengming,Ge, Xianping,Fu, Hongtuo,Zhu, Jian,Xuan, Fujun,Zhang, Shiyong.

[15]THE METALLOTHIONEIN GENE FROM THE ORIENTAL RIVER PRAWN MACROBRACHIUM NIPPONENSE (DE HAAN, 1849): CHARACTERIZATION AND EXPRESSION IN RESPONSE TO HYPDXIA AND REOXYGENATION. Sun, Shengming,Gu, Zhimin,Fu, Hongtuo,Zhu, Jian.

[16]Contribution of sediment oxygen demand to hypoxia development off the Changjiang Estuary. Zhang, Haiyan,Zhao, Liang,Sun, Yao,Wang, Jianing,Wei, Hao.

[17]Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi. Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei.

[18]Bacterial growth efficiency in a partly eutrophicated bay of South China Sea: Implication for anthropogenic impacts and potential hypoxia events. Song, Xing-Yu,Liu, Hua-Xue,Zhong, Yu,Tan, Ye-Hui,Qinl, Geng,Li, Kai-Zhi,Shen, Ping-Ping,Huang, Liang-Min,Wang, You-Shao,Song, Xing-Yu,Zhong, Yu,Wang, You-Shao,Liu, Hua-Xue.

[19]Molecular cloning, characterization, and expression analysis of p53 from the oriental river prawn, Macrobrachium nipponense, in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Gu, Zhimin,Xuan, Fujun.

[20]Studies on resistance characteristic and cDNA sequence conservation of transferrin from crucian carp, Carassius auratus. Long, Hua,Yu, Qi-Xing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台