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Establishment of uracil auxotrophic dikaryotic strains of Lentinula edodes by crossbreeding

文献类型： 外文期刊

作者： Zhou, Chenli 1 ; Xi, Liping 1 ; Mao, Wenjun 1 ; Wan, Jianing 1 ; Li, Yan 1 ; Wang, Ying 1 ; Bao, Dapeng 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Lab Edible Fungi Genet, 12th Bldg,1000 Jinqi Rd, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Key Lab Appl Mycol Resources & Utilizat, Minist Agr, 12th Bldg,1000 Jinqi Rd, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, 12th Bldg,1000 Jinqi Rd, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Inst Edible Fungi, 12th Bldg,1000 Jinqi Rd, Shanghai 201403, Peoples R China

关键词： Lentinula edodes;uracil auxotroph;dikaryon

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

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收录情况： SCI

摘要： The uracil auxotrophic monokaryotic strain 423-9 of Lentinula edodes was crossed with nine monokaryons (cro2-2-9, W66-1, xd2-3-2, QingKe 20A, 241-1-1, 9015-1, L66-2, 241-1-2, and Qing 23A) derived from wild type strains of L. edodes. Nine dikaryotic hybrids were established from these crosses. These hybrids were fruited and 496 single spore isolates were obtained. Among these single spore isolates, 166 were identified as monokaryons under a microscope. We screened these monokaryons on selective medium and obtained 19 uracil auxotrophic monokaryons. By using the Monkaryon-monkaryon crossing method among the uracil auxotrophic monokaryons, 56 uracil auxotrophic dikaryotic strains were established on selective medium. These dikaryotic strains were unable to grow on minimal medium without uracil and exhibited slow growth rates on PDA plates compared to the wild type strain. The uracil auxotrophic dikaryotic strains also showed more vigorous growth on sawdust cultivation medium containing uracil than that without uracil. The fruiting tests showed that they formed normal fruiting bodies on the sawdust medium containing uracil. The results show that the uracil auxotrophic dikaryotic strain of L. edodes could be produced by mating, and will provide a valuable resource for future genetic studies and for spawn protection and identification.

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