您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

In Vivo Determination of Reduced Thiols in Rat Cerebellum Paraflocculus Following Salicylate-Induced Tinnitus by Fluorescence

文献类型： 外文期刊

作者： Zhao, Lingzhi 1 ; Li, Zhao 2 ; Zhao, Liu 3 ; Zhang, Chenxiao 2 ;

作者机构： 1.Xian Med Coll, Dept Pharm, Inst Pharmacol, Xian, Shaanxi Provinc, Peoples R China

2.Shaanxi Normal Univ, Dept Chem & Chem Engn, Lab Analyt Chem Life Sci Shaanxi Prov, Xian, Peoples R China

3.Beijing Res Ctr Agr Stand & Testing, Beijing, Peoples R China

关键词： Cerebellum;fluorescence;paraflocculus;reduced biothiols;salicylate-induced tinnitus

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The increasing number of patients suffering from tinnitus may be explained by an imbalance in the antioxidant defense system and reactive oxygen species formation, suggesting the importance of the redox homeostasis in this condition. Endogenous biothiols play important roles in maintaining redox homeostasis. Hence, to understand the role of biothiols in the pathological process of tinnitus, this study demonstrates an in vivo method for monitoring the concentrations of biothiols in the paraflocculus of the rat cerebellum during tinnitus induced by the injection of salicylate. Resorufin-based fluorescent probe 1 was used as the selective probe with in vivo microdialysis. Probe 1 was premixed with microdialysates from the paraflocculus of the rat cerebellum and transferred into a cuvette for continuous-flow fluorescence. A linear relationship between the fluorescence and the concentrations of cysteine, homocysteine, and glutathione was from 1 to 15 mu M, with detection limits of 128, 148, and 183 nM, respectively. The basal level of total reduced biothiols in the paraflocculus of the rat cerebellum microdialysate was determined to be 10.62. 1.34 mu M based on the calibration curve for homocysteine. The injection of sodium salicylate into the animals significantly decreased the reduced biothiol concentrations in the paraflocculus of the rat cerebellum from 60 min, reaching 39.5. 6.20% of the basal level. In contrast, at 120, 180, and 300 min, the concentrations of reduced biothiols were 55.73. 9.35, 58.26. 9.56, and 91.69. 6.91% of the basal level, respectively. These results imply that the decrease in reduced biothiols in the paraflocculus of the rat cerebellum may be associated with salicylate-induced tinnitus. This study offers a new platform for in vivo monitoring of reduced biothiols in the paraflocculus of the rat cerebellum following salicylate-induced tinnitus and may be useful for the investigation of this condition.

  • 相关文献

[1]A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li,Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li. 2016

[2]Superior fluorescent probe for detection of potassium ion. Chen, Zhengbo,Tan, Lulu,Hu, Liangyu,Luan, Yunxia.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台