您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of Compounds with Tumor-Cell Proliferation Inhibition Activity from Mushroom (Phellinus baumii) Mycelia Produced by Solid-State Fermentation

文献类型： 外文期刊

作者： Zhang, Henan 1 ; Shao, Qian 1 ; Wang, Wenhan 1 ; Zhang, Jingsong 1 ; Zhang, Zhong 1 ; Liu, Yanfang 1 ; Yang, Yan 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South, Inst Edible Fungi,Minist Agr, Shanghai 201403, Peoples R China

2.Shanghai Acad Ag

关键词： Phellinus baumii;solid-state;Extracts;compound isolation;proliferation inhibition

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The inhibition of tumor-cell proliferationbyan organicsolvent extract from the solid-state fermentation of Phellinus baumii mycelia inoculated in rice medium was investigated in vitro. The active compounds inhibiting tumor-cell proliferation were characterized. Results revealed that all (petroleum ether, chloroform, ethyl acetate, and butanol) fractions inhibited tumor-cell proliferation in a dose-dependent fashion. The ethyl acetate extract had the highest inhibitory effecton tumor-cell proliferation, and the butanol fraction had the lowest. Six compounds were isolated and purified from the ethyl acetate extract of P. baumii mycelia by the tandem application of silica-gel column chromatography (SGCC), high-speed countercurrent chromatography (HSCCC), and preparative HPLC. These compounds were identified by NMR and electrospray ionization-mass spectrometry (ESI-MS) spectroscopic methods as ergosterol (RF1), ergosta-7,22-dien-3 beta-yl pentadecanoate (RF3), 3,4-dihydroxy benzaldehyde(RF6), inoscavinA (RF7), baicalein(RF10), and 24-ethylcholesta-5,22-dien-3 beta-ol (RF13). To further clarify the activity of these compounds, the cell-proliferation-inhibition tests of these compounds on various tumor cells were carried out and evaluatedin vitro. Results suggested that compounds RF6, RF7, and RF10 had potent inhibition effects on the proliferation of a series of tumor cell lines, including K562, L1210, SW620, HepG2, LNCaP, and MCF-7cells. These findings indicated that P. baumii mycelia produced by solid-state fermentation in rice canbe used to obtain active compounds with the ability to inhibittumor-cell proliferation.

  • 相关文献

[1]Cultivation and bioactivity assessment of Grifola frondosa fruiting bodies on olive oil press cakes substrates. Gregori, Andrej,Klinar, Dusan,Svagelj, Mirjan,Berovic, Marin,Liu, Yanfang,Zhang, Jingsong,Pohleven, Franc.

[2]Biological Activities of Ethanol Extracts of Phellinus baumii (Higher Basidiomycetes) Obtained by Different Fermentation Methods. Shao, Qian,Yang, Yan,Li, Tingting,Feng, Jie,Liu, Yanfang,Yan, Mengqiu,Zhu, Lina,Tang, Chuanhong,Shao, Qian,Yang, Yan,Li, Tingting,Feng, Jie,Liu, Yanfang,Yan, Mengqiu,Zhu, Lina,Tang, Chuanhong,Shao, Qian,Li, Tingting.

[3]Preparative Isolation of 5 Antioxidant Constituents from the Medicinal Mushroom Phellinus baumii (Agaricomycetes) by High-Speed Countercurrent Chromatography and Preparative High-Performance Liquid Chromatography. Ye, Libin,Zhang, Zhong,Yang, Yan,Ye, Libin,Zhang, Zhong,Yang, Yan,Ye, Libin,Zheng, Hongjian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台