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Comparative metabolic profiling of four transgenic maize lines and two non-transgenic maize lines using high-performance liquid chromatography mass spectrometry

文献类型： 外文期刊

作者： Hao, Wenyuan 1 ; Li, Feiwu 2 ; Yan, Wei 2 ; Li, Congcong 2 ; Hao, Dongyun 1 ;

作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, 50 Hexing Rd, Harbin 150080, Heilongjiang, Peoples R China

2.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Jilin, Peoples R China

关键词： Bacillus thuringiensis;Pest-resistant transgenic maize;Metabolomics analysis;Purine and glutathione metabolism

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

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收录情况： SCI

摘要： We compared metabolic changes in transgenic maize lines (differing in genetic backgrounds and exogenous genes insertion) with non-transgenic counterparts. At the corn seedling stage, entirely expanding leaves were obtained from maize lines 1 (transgenic maize SK12-5; overexpressing cry1Ab/cry2Aj; parental maize ZD958), 2 (transgenic maize IE034; overexpressing cry1Ie; parental maize ZD958), 3 (transgenic maize Bt799; overexpressing cry1Ac-M; parental maize Z58), 4 (transgenic maize Bt799; overexpressing cry1Ac-M; parental maize ZD958), 5 (non-transgenic maize Z58), and 6 (non-transgenic maize ZD958). For each line, six biological replicates were prepared; each contained an entirely expanded leaf at the top of the plant. Metabolites were identified with high-performance liquid chromatography mass spectrometry in three comparison groups (4 transgenic maize lines vs. their corresponding controls, line 3 vs. 4, and transgenic vs. non-transgenic maize). Top 200 cationic and top 200 anionic metabolites with higher variable importance in projection value were chosen for principal component analysis, followed by pathway enrichment analysis. Annotation was possible for 227 of 400 metabolites. Twelve up- and 26 down-regulated overlapping metabolites were identified in line 1 vs. 6, line 2 vs. 6, and line 4 vs. 6 comparison groups. Altered metabolites significantly enriched in purine and glutathione metabolism pathways, but no pathways were enriched in line 3 vs. 4. Comparing transgenic and non-transgenic maize lines revealed 59 up- and 37 down-regulated metabolites, which were also significantly enriched in purine and glutathione metabolism pathways. Therefore, transgenic Bt maize differed metabolically from non-transgenic maize. Additionally, purine and glutathione metabolism pathways may be important in the transgenic maize metabolism.

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