您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Differential Protein Analysis of IPEC-J2 Cells Infected with Porcine Epidemic Diarrhea Virus Pandemic and Classical Strains Elucidates the Pathogenesis of Infection

文献类型： 外文期刊

作者： Lin, Huixing 1 ; Li, Bin 2 ; Chen, Lei 1 ; Ma, Zhe 1 ; He, Kongwang 2 ; Fan, Hongjie 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： differential protein expression;comparative proteomics;porcine epidemic diarrhea virus;virulence

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea (PED) re-emerged in China in late 2010 and has now become widespread. Accumulated evidence indicates that this large-scale outbreak of diarrhea Was caused by variants of the highly virulent porcine epidemic diarrhea virus (PEDV). A pandemic PEDV YC2014 strain (YC2014) was isolated from clinical samples. 6 An iTRAQ-based comparative quantitative proteomic study of IPEC-J2 cells infected with YC2014 and a classical CV777 strain (CV777) was performed to determine the differences between pandemic and classical PEDV strain infection. Totals of 353 and 299 differentially expressed proteins were identified upon YC2014 and CV777 infection, respectively. The canonical pathways and functional networks involved in both PEDV infections were analyzed. The results indicated that the PEDV suppressed protein synthesis of IPEC-J2, cells through down-regulation of the PI3K-AKT/mTOR signaling pathways. Infection with YC2014 could activate the JAK-STAT signaling pathway and the NF-kappa B pathway more intensively than CV777. YC2014 could activate NF-kappa B pathway more intensively than CV777. On the basis of differentially expressed proteins, we propose that PEDV might disrupt apoptosis and may elicit stronger inflammatory cascades as well. This study might contribute to an understanding of the pathogenesis of PEDV infection and aid in the development of effective preventive and control vaccines.

  • 相关文献

[1]Poly (D,L-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in piglets. Li, Bin,Yu, Zhengyu,Sun, Bing,Xu, Xiangwei,Fan, Baochao,Guo, Rongli,He, Kongwang,Du, Luping,Yuan, Wanzhe. 2017

[2]XocR, a LuxR solo required for virulence in Xanthomonas oryzae pv. oryzicola. Xu, Huiyong,Zhao, Yancun,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan. 2015

[3]Determination of the complete genomic sequence and molecular biological analysis of Soybean mosaic virus. Zhang, Hongyun,Zhi, Haijian,Zhang, Siwei,Zhao, Lin,Cui, Xiaoyan,Chen, Xin. 2012

[4]Bacteriocinogenic Enterococcus faecium inhibits the virulence property of Listeria monocytogenes. Ye, Keping,Huang, Yan,Liu, Jia,Liu, Mei,Zhou, Guanghong,Zhang, Xinxiao. 2018

[5]Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum. Liu, Xin,Xu, Jianhong,Wang, Jian,Ji, Fang,Yin, Xianchao,Shi, Jianrong.

[6]Baseline sensitivity of natural population and resistance risk of Peronophythora litchii to four novel QoI fungicides. Zhou, Yuxin,Liu, Pengfei,Zhang, Yu,Meng, Qingxiao,Liu, Xili,Zhou, Yuxin,Chen, Lei,Hu, Jian,Li, Bin,Si, Naiguo,Liu, Changling.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台