您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Physiological investigation of C-4-phosphoenolpyruvate-carboxylase-introduced rice line shows that sucrose metabolism is involved in the improved drought tolerance

文献类型： 外文期刊

作者： Zhang, Chen 1 ; Li, Xia 1 ; He, Yafei 1 ; Zhang, Jinfei 1 ; Yan, Ting 1 ; Liu, Xiaolong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food & Crops, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

关键词： Drought;Phosphoenolpyruvate carboxylase;Transgenic rice;Sugar;Carbohydrate metabolism;Gene expression regulation

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We compared the drought tolerance of wild-type (WT) and transgenic rice plants (PC) over-expressing the maize C-4 PEPC gene, which encodes phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) gene, and evaluated the roles of saccharide and sugar-related enzymes in the drought response. Pot-grown seedlings were subjected to real drought conditions outdoors, and the yield components were compared between PC and untransformed wild-type (WT) plants. The stable yield from PC plants was associated with higher net photosynthetic rate under the real drought treatment. The physiological characters of WT and PC seedlings under a simulated drought treatment (25% (w/v) polyethylene glycol 6000 for 3 h; PEG 6000 treatment) were analyzed in detail for the early response of drought. The relative water content was higher in PC than in WT, and PEPC activity and the C-4-PEPC transcript level in PC were elevated under the simulated drought conditions. The endogenous saccharide responses also differed between PC and WT under simulated drought stress. The higher sugar decomposition rate in PC than in WT under drought analog stress was related to the increased activities of sucrose phosphate synthase, sucrose synthase, acid invertase, and neutral invertase, increased transcript levels of VIN1, CIN1, NIN1, SUT2, SUT4, and SUB, and increased activities of superoxide dismutase and peroxidase in the leaves. The greater antioxidant defense capacity of PC and its relationship with saccharide metabolism was one of the reasons for the improved drought tolerance. In conclusion, PEPC effectively alleviated oxidative damage and enhanced the drought tolerance in rice plants, which were more related to the increase of the endogenous saccharide decomposition. These findings show that components of C4 photosynthesis can be used to increase the yield of rice under drought conditions. (C) 2017 Elsevier Masson SAS. All rights reserved.

  • 相关文献

[1]Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane. Shen, W. J.,Chen, G. X.,Xu, J. G.,Jiang, Y.,Liu, L.,Gao, Z. P.,Ma, J.,Lv, C. F.,Chen, X.,Chen, T. H..

[2]Improved oxidative tolerance in suspension-cultured cells of C-4-pepctransgenic rice by H2O2 and Ca2+ under PEG-6000. Qian, Baoyun,Li, Xia,Liu, Xiaolong,Wang, Man,Qian, Baoyun,Liu, Xiaolong,Qian, Baoyun,Li, Xia,Liu, Xiaolong,Wang, Man. 2015

[3]Improved short-term drought response of transgenic rice over-expressing maize C-4 phosphoenolpyruvate carboxylase via calcium signal cascade. Liu, Xiaolong,Li, Xia,Yan, Ting,Zhang, Jinfei,Liu, Xiaolong,Li, Xia,Yan, Ting,Dai, Chuanchao,Zhou, Jiayu.

[4]Exogenous ATP enhance signal response of suspension cells of transgenic rice (Oryza sativa L.) expressing maize C-4 -pepc encoded phosphoenolpyruvate carboxylase under PEG treatment. Huo, K.,Li, X.,He, Y. F.,Wei, X. D.,Zhao, C. F.,Wang, C. L.,Huo, K.,Lu, W..

[5]Enhanced drought tolerance in transgenic rice over-expressing of maize C-4 phosphoenolpyruvate carboxylase gene via NO and Ca2+. Qian, Baoyun,Li, Xia,Liu, Xiaolong,Chen, Pingbo,Ren, Chengang,Qian, Baoyun,Liu, Xiaolong,Dai, Chuanchao.

[6]Characteristics of carbon assimilation and chlorophyll fluorescence in C_4 photosynthetic enzymes transgenic rice. D-M Jiao,X-Q Huang,X Li,W Chi,T-Y Kuang,Q-D Zhang,MSB Ku. 2001

[7]Characteristics of carbon assimilation and tolerance to photo-oxidation in transgenic rice expressing C_4 photosynthesis enzymes. D-M Jiao,X-Q Huang,X Li,W Chi,T-Y Kuang,Q-D Zhang,MSB Ku. 2001

[8]Evaluation of sugar, free amino acid, and organic acid compositions of different varieties of vegetable soybean (Glycine max [L.] Merr). Song, Jiangfeng,Gu, Zhenxin,Song, Jiangfeng,Liu, Chunquan,Li, Dajing,Liu, Chunquan. 2013

[9]The endogenous plant hormones and ratios regulate sugar and dry matter accumulation in Jerusalem artichoke in salt-soil. Li, Lingling,Shao, Tianyun,Yang, Hui,Chen, Manxia,Gao, Xiumei,Long, Xiaohua,Liu, Zhaopu,Shao, Hongbo,Shao, Hongbo,Rengel, Zed.

[10]Characteristics of CO2 exchange and chlorophyll fluorescence of transgenic rice with C-4 genes. Huang, XQ,Jiao, DM,Chi, W,Ku, MSB. 2002

[11]The characteristics of CO2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice. Jiao, DM,Li, X,Huang, XQ,Wei, C,Kuang, TY,Maurice, KSB. 2001

[12]CO2 exchange and chlorophyll fluorescence of phosphoenolpyruvate carboxylase transgenic rice pollen lines. Ling, Li-Li,Lin, Hong-Hui,Ji, Ben-Hua,Jiao, De-Mao. 2006

[13]RNAi-mediated transgenic rice resistance to Rice stripe virus. Wang Biao,Lei Yang,Dai Yu-hua,He Wen,Liang Chun,Wang Xi-feng,Guo Cheng,Liang Chun,Zhou Tong,Liang Chun. 2016

[14]A limited photosynthetic C-4-microcycle and its physiological function in transgenic rice plant expressing the maize PEPC gene. Ji, BH,Zhu, SQ,Jiao, DM. 2004

[15]Response of Gas Exchange and Water Use Efficiency to Light Intensity and Temperature in Transgenic Rice Expressing PEPC and PPDK Genes. Jiao De-mao,Zhang Bian-jiang,Chen Quan-zhan,Hua Chun,Zhou Feng,Zhou Quan-chen. 2009

[16]Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C(4) photosynthesis enzymes. Jiao, DM,Huang, XQ,Li, X,Chi, W,Kuang, TY,Zhang, QD,Ku, MSB,Cho, DH. 2002

[17]Physiological and metabolic enzymes activity changes in transgenic rice plants with increased phosphoenolpyruvate carboxylase activity during the flowering stage. Xia, Li,Cao, Wang,Xia, Li.

[18]Resistance strategies of Phragmites australis (common reed) to Pb pollution in flood and drought conditions. Zhang, Na,Zhang, Zhenhua,Zhang, Na,Chen, Jing,Zhang, Na,Zhang, Jinwei,Mu, Chunsheng,Li, Zhiqiang. 2018

[19]Genotypically Identifying Wheat Mesophyll Conductance Regulation under Progressive Drought Stress. Olsovska, Katarina,Brestic, Marian,Shao, Hong Bo,Olsovska, Katarina,Kovar, Marek,Brestic, Marian,Zivcak, Marek,Slamka, Pavol,Shao, Hong Bo. 2016

[20]Recent progress in drought and salt tolerance studies in Brassica crops. Zhang, Xuekun,Lu, Guangyuan,Long, Weihua,Zou, Xiling,Li, Feng,Long, Weihua,Nishio, Takeshi.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台