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Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.)

文献类型： 外文期刊

作者： Wang, He 1 ; Fan, Mingxia 1 ; Wang, Guohong; Zhang, Chunyu 2 ; Shi, Lei; Wei, Zhengyi; Ma, Wenjuan 2 ; Chang, Ji 1 ;

作者机构： 1.Shenyang Agr Univ, Agron Coll, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

2.Shenyang Agr Univ, Biotechnol & Biosci Coll, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

3.Liaoning Acad Agr Sci, Corn Res Inst, 84 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

关键词： pollen-specific promoter;maize;ZmSTK2_USP gene;PCR;GUS activity

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP- GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

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