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The involvement of cathepsin F gene (CTSF) in turbot (Scophthalmus maximus L.) mucosal immunity

文献类型: 外文期刊

作者: Gao, Chengbin 1 ; Fu, Qiang 1 ; Su, Baofeng 3 ; Song, Huanhuan 1 ; Zhou, Shun 1 ; Tan, Fenghua 2 ; Li, Chao 1 ;

作者机构: 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China

2.Qingdao Agr Univ, Sch Int Educ & Exchange, Qingdao 266109, Peoples R China

3.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin 150070, Peoples R China

4.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Key Lab Freshwater Aquat Biotechnol & Breeding, Minist Agr, Harbin 150070, Peoples R China

5.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200062, Peoples R China

关键词: CTSF;Mucosal surfaces;Turbot;Vibrio anguillarum;Streptococcus iniae;Whole-cell vaccine

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 2020影响因子:4.581; 五年影响因子:4.851 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Cathepsin F (CTSF) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated N-terminal pro-region, which contains a cystatin domain. CTSF likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (MHC) class II. In this regard, we identified the CTSF gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. We also determined the expression patterns of CTSF in mucosal tissues after vaccinated with the formalin-inactivated V. vulnificus whole-cell vaccine. Briefly, turbot CTSF gene showed the closest relationship with that of Paralichthys olivaceus in phylogenetic analysis. And CTSF was ubiquitously expressed in all tested tissues with the highest expression level in gill. In addition, CTSF gene showed different expression patterns following different bacterial challenge. The significant quick regulation of CTSF in mucosal surfaces against infection indicated its roles in mucosal immunity. Functional studies should further characterize avail utilization of CTSF function to increase the disease resistance of turbot in maintaining the integrity of the mucosal barriers against infection and to facilitate selection of the disease resistant family/strain in turbot. (C) 2017 Elsevier Ltd. All rights reserved.

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