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The biological features and genetic diversity of novel fish rhabdovirus isolates in China

文献类型： 外文期刊

作者： Fu, Xiaozhe 1 ; Lin, Qiang 2 ; Liang, Hongru 2 ; Liu, Lihui 2 ; Huang, Zhibin 3 ; Li, Ningqiu; Su, Jianguo 1 ;

作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Shanxi Key Lab Mol Biol Agr, Yangling 712100, Shanxi, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr,Key Lab Aquat Anim Immune Technol, Guangzhou 510380, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Mini

关键词： Fish rhabdovirus;Biological features;Genetic diversity

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 novel viruses from diseased fish collected from China including SCRV-QY, SCRV-SS, SCRV-GM, CmRV-FS, MsRV-SS, OmbRV-JM. The typical clinical symptom of diseased fish was hemorrhaging. Efficient propagation of these isolates in a Chinese perch brain cell line was determined by means of observation of cytopathic effect, RT-PCR and electron microscopy. Sequence alignment and phylogenetic analysis of the complete G protein sequences revealed that these isolates were clustered into one monophyletic lineage belonging to the species Siniperca chuatsi rhabdovirus.

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