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The development of a sensitive droplet digital PCR for quantitative detection of porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

作者： Yang, Qi 1 ; Xi, Jun 2 ; Chen, Xinxin 3 ; Hu, Sihong 1 ; Chen, Nan 1 ; Qiao, Songlin 3 ; Wan, Shaogui 1 ; Bao, Dengke 1 ;

作者机构： 1.Henan Univ, Pharmaceut Coll, Kaifeng, Henan, Peoples R China

2.Henan Univ Technol, Sch Food Sci & Technol, Zhengzhou, Henan, Peoples R China

3.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China

关键词： Porcine reproductive and respiratory syndrome virus;Droplet digital PCR;Detection

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease, resulting in important economic losses in pig farming. Prompt detection of PRRS virus (PRRSV) in the field samples is important for effective PRRS control. Droplet digital PCR (ddPCR) is a novel PCR technology, which offers good precision and direct quantification without using calibration curves. In this study, we established a ddPCR system for the sensitive and accurate quantification of PRRSV. Specificity of the assay was determined by the failure of amplification of other relevant viruses. Quantitative linearity, sensitivity and accuracy of ddPCR were compared to those of real time PCR for PRRSV testing. Both methods showed a high degree of linearity (R-2 = similar to 1) and quantitative correlation, although ddPCR showed somewhat higher sensitivity than real time PCR. Collectively, our findings indicate that ddPCR might offer improved analytical sensitivity and specificity for PRRSV measurements. (C) 2017 Elsevier B.V. All rights reserved.

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