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Cloning, characterization, expression analysis and RNAi of Retinoblastoma-like gene from black tiger shrimp (Penaeus monodon)

文献类型： 外文期刊

作者： Xie, Bobo 1 ; Wang, Pengfei 1 ; Zhao, Chao 1 ; Qiu, Lihua 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai, Peoples R China

3.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China

4.South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ctr, Sanya, Peoples R China

关键词： mRNA expression;ovarian development;Penaeus monodon;retinoblastoma;RNAi;RT-qPCR

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Retinoblastoma (Rb) is a multifunctional regulator involved in several key cellular processes, such as cell cycle control, cell differentiation, tumorigenesis and senescence. In this study, an Rb-like gene, PmRBL, was cloned from black tiger shrimp (Penaeus monodon). The full-length cDNA sequence of PmRBL is 4,069bp with an open reading frame of 3,243bp, which encodes 1,080 amino acids. Quantitative real-time PCR (qRT-PCR) analysis indicated that PmRBL was highly expressed in the gills, hepatopancreas and ovaries of P.monodon. The highest PmRBL expression levels were observed in stage III of the ovarian development in P.monodon. RNA interference experiments were conducted to examine the expression profiles of PmRBL, PmCDK2 and PmCyclin E. The knockdown of PmRBL in the ovary and hepatopancreas by dsRNA-RBL was successful. After dsRNA-RBL was injected into the shrimp, the relative expression levels of PmCDK2 and PmCyclin E were upregulated at 12-72hr in the ovaries and hepatopancreas. The localization and level of PmRBL expression in the ovary and hepatopancreas were investigated through insitu hybridization, which revealed consistent results with those of qRT-PCR. Therefore, PmRBL, PmCDK2 and PmCyclin E may be involved in vitellogenin synthesis and ovarian maturation in P.monodon.

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