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Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System

文献类型： 外文期刊

作者： Sun, Lichang 1 ; He, Tao 1 ; Zhang, Lili 1 ; Pang, Maoda 1 ; Zhang, Qiaoyan; Zhou, Yan 2 ; Bao, Hongduo; Wang, Ran;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Standard Agroprod Safet, Minist Agr,Key Lab Food Qual & Safety Jiangsu Pro, State Key Lab Breeding Base,Inst Food Qual & Safe, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Control Technol & Standard Agroprod Safet, Minist Agr,Key Lab Food Qual & Safety Jiangsu Pro, State Key Lab Breeding Base,Inst

关键词： mcr-1;knockout;E. coli;CRISPR/Cas9 system;BMAP-27

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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收录情况： SCI

摘要： The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas:: mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

  • 相关文献

[1]Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows. He, Tao,Wei, Ruicheng,Zhang, Lili,Sun, Lichang,Pang, Maoda,Wang, Ran,Wang, Yang.

[2]Accumulation of antibiotics and heavy metals in meat duck deep litter and their role in persistence of antibiotic-resistant Escherichia coli in different flocks on one duck farm. Zhang, X. T.,Ji, X.,Zou, X. T.,Yao, W.,Lin, Y.,Zhao, W.,Shi, Z. D.,Gu, H. R.,Gong, J. S..

[3]Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases. Huang, Xian-Ju,Zhang, Hong-Xiao,Xiong, Kai,Qin, Ling,Liu, Honglin,Wang, Huili. 2014

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