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Polyphenol Profiles and Antioxidant Properties of Ethanol Extracts from Osmanthus Fragrans (Thunb.) Lour. Flowers

文献类型： 外文期刊

作者： Li, Hong-Ling 1 ; Chai, Zhi 1 ; Shen, Garry X. 3 ; Li, Chun-Yang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Farm Prod Proc, Dept Funct Food & Bioact Cpds, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

3.Univ Manitoba, Dept Internal Med, Winnipeg, MB R3E 3P4, Canada

4.Univ Manitoba, Dept Human Nutr Sci, Winnipeg, MB R3E 3P4, Canada

关键词： ethanol extracts of Osmanthus fragrans flowers;antioxidant;polyphenol profiles;cultured endothelial cells

期刊名称：POLISH JOURNAL OF FOOD AND NUTRITION SCIENCES （ 影响因子：2.111； 五年影响因子：2.672 ）

ISSN：

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收录情况： SCI

摘要： This study evaluated the antioxidant activity of ethanol extracts of Osmanthus fragrans (Thunb.) Lour. flowers (EOF) and identified phenolic compounds in EOF using liquid chromatography-mass spectrometry. Nine compounds, 3-O-caffeoylquinic acid, caffeic acid 4-O-glucoside, salidroside, 5-O-coumaroylquinic acid, 4-O-coumaroylquinic acid, acteoside, ligustroside, fucosterol and arjunolic acid were identified. To our knowledge, caffeic acid 4-O-glucoside, 5-O-coumaroylquinic acid and 4-O-coumaroylquinic acid have not been detected in EOF. In vitro antioxidant activity analysis demonstrated that EOF possessed strong DPPH and ABTS radicals scavenging activity with EC50 values at 0.26 +/- 0.06 mg/mL and 0.36 +/- 0.01 mg/mL, respectively, and reducing power with Ab(0.5) value at 13.04 +/- 0.16 mu g/mL. The value of oxygen radical absorbance capacity (ORAC) was 333.23 +/- 13.39 mu mol Trolox/g. Antioxidant activity assay in human umbilical vein endothelial cells (HUVEC) showed that the activity of superoxide dismutase (SOD) was significantly improved and the reactive oxygen species (ROS) was removed effectively from cells when treated with EOF of 300 similar to 3000 mu g/L.

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