Improvement of the Thermostability of Xylanase from Thermobacillus composti through Site-Directed Mutagenesis
文献类型： 外文期刊
作者： Tian, Yong-Sheng 1 ; Xu, Jing 1 ; Lei-Chen 1 ; Fu, Xiao-Yan 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
2.Shanghai Ruifeng Agr Sci & Technol Co Ltd, Shanghai 201106, Peoples R China
3.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
关键词： Xylanase thermostability;Thermobacillus composti;disulfide bridge;structure
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
ISSN： 1017-7825
年卷期： 2017 年 27 卷 10 期
页码：
收录情况： SCI
摘要： Thermostability is an important property of xylanase because high temperature is required for its applications, such as wood pulp bleaching, baking, and animal feedstuff processing. In this study, XynB from Thermobacillus composti, a moderately thermophilic gram-negative bacterium, was modified via site-directed mutagenesis (based on its 3D structure) to obtain thermostable xylanase, and the properties of this enzyme were analyzed. Results revealed that the half-life of xylanase at 65 degrees C increased from 10 to 50 min after a disulfide bridge was introduced between the a-helix and its adjacent beta-sheet at S98 and N145. Further mutation at the side of A153E named XynB-CE in the C-terminal of this a-helix enhanced the half-life of xylanase for 60 min at 65 degrees C. Therefore, the mutant may be utilized for industrial applications.
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