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GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty

文献类型： 外文期刊

作者： Yang, Kun 1 ; Yan, Qiang 1 ; Wang, Yi 1 ; Peng, Hao 3 ; Jing, Maofeng 1 ; Dou, Daolong 1 ;

作者机构： 1.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Coll Plant Protect, Key Lab Plant Immun, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Ind Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210095, Peoples R China

3.Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA

关键词： Genetic engineering; Disease resistance; Hydrogen peroxide; Phytophthora; Polyamine oxidase; Soybean

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）

ISSN： 2096-5362

年卷期： 2022 年 4 卷 1 期

页码：

收录情况： SCI

摘要： Plant immunity is activated upon perception of pathogens and often affects growth when it is constitutively active. It is still a challenge to balance plant immunity and growth in disease resistance breeding. Here, we demonstrated that soybean (Glycine max) polyamine oxidase (GmPAO) confers resistance to multiple Phytophthora pathogens, but has no obvious adverse impact on agronomic traits. GmPAO produces H2O2 by oxidizing spermidine and spermine. Phytophthora sojae induces an increase in these two substrates, and thus promotes GmPAO-mediated polyamine catabolism specifically during infection. Interestingly, we found that the two substrates showed higher accumulation in transgenic soybean lines overexpressing GmPAO than in WT and CK after inoculation with P. sojae to ensure H2O2 production during infection, rather than directly inhibit P. sojae. In these transgenic soybean plants, the significantly enhanced resistance to different P. sojae isolates was achieved; PAMP-induced H2O2 accumulation was enhanced by GmPAO overexpression. Moreover, transient expression of GmPAO also significantly improved Nicotiana benthamiana resistance to Phytophthora capsici and Phytophthora parasitica in agroinfiltration assays. Our results provide a novel approach to allow rapid defense responses in plants upon pathogen infection while minimizing growth penalties under normal conditions, with a clear mechanism in which plant promotes H2O2 production via pathogen-activated substrates.

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