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Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats

文献类型： 外文期刊

作者： Zhu, Yumin 1 ; Yu, Xiaoming 1 ; Zhang, Yuanshu 3 ; Ni, Yanyan 4 ; Si, Fusheng 1 ; Yu, Ruisong 1 ; Dong, Shijuan 1 ; Huang 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai, Peoples R China

2.Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China

3.Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China

4.Virginia Polytech Inst & State Univ, Coll Vet Med, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA

5.Virginia Polytech Inst & State Univ, Coll Vet Me

关键词： Hepatitis E virus (HEV);Infectious clone;Genotype 4;Rat animal model

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2013 年 166 卷 3-4 期

页码：

收录情况： SCI

摘要： A cDNA clone of a genotype 4 swine hepatitis E virus (HEV) strain (SAAS-FX17), identified in Shanghai, has been constructed. Capped RNA transcripts were prepared in vitro and shown to be replication-competent in Huh7 cells. Sprague-Dawley (SD) rats administered the RNA transcripts by intrahepatic inoculation developed active infections as evidenced by fecal virus shedding and sero-conversion to anti-HEV. The former was first detected between 23 and 30 days post-inoculation (dpi) and persisted until 45 dpi. Sera of rats inoculated with RNA transcripts became anti-HEV positive between 30 and 40 dpi, and reverted to anti-HEV negative at 52 dpi. Our data indicate for the first time that intrahepatic inoculation of rats with RNA transcripts of an HEV cDNA clone may serve as an alternative animal model for HEV research. (C) 2013 Elsevier B.V. All rights reserved.

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