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Isolation an antimicrobial action of endophytic fungi from sophora flavescens and effects on microorganism circumstances in soil

文献类型： 外文期刊

作者： He, Lu 1 ; Liu, Na 2 ; Wang, Yong 1 ; Xu, Hong Bo 1 ; Yu, Na 1 ;

作者机构： 1.Univ Sci & Technol Liaoning, Coll Chem Engn, Anshan 114051, Peoples R China

2.Shanghai Acad Agr Sci, Informat Res Inst, Shanghai 201403, Peoples R China

关键词： endophytic fungi;biological activity;soil microorganism circumstances;Sophora flavescens

期刊名称：2013 INTERNATIONAL SYMPOSIUM ON ENVIRONMENTAL SCIENCE AND TECHNOLOGY (2013 ISEST)

ISSN： 1878-0296

年卷期： 2013 年 18 卷

页码：

收录情况： SCI

摘要： The aim of this study was to isolate the endophytic fungi from Sophora flavescens and define its antimicrobial action and structure. The effects of this active substance on soil microorganism circumstances were also conducted. Column chromatography and freeze drying were used to isolate and purify the antimicrobial substance. TLC biological autoradiography was applied to trace. HPLC method was employed to measure the purity. Analysis of the structure used H-1-NMR, C-13-NMR and LC-MS methods. Plate method was applied to measure the effect on microorganism circumstances. The results showed that after isolating and purifying the fermentation liquor of BS001., the structure of bacteriostatic active composition was 6,7-(2'E)dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-1,4-dione, which was identified by spectroscopy. The substance could increase the number of bacteria and fungi while decreased the number of antinomies in soil. A new antimicrobial substance 6,7-(2'E)dibuteny1-5,8 -dihydroxy- (Z)- cyclooct - 2ene-1,4-dione was extracted from fermenting liquor of BS001 which was an endophytic fungi of Sophora flavescens. It could promote the beneficial flora but detrimental flora. "this conclusion provides exploiture foreground for biopharmaceuticals and biopesticide. (C) 2013 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license

  • 相关文献

[1]Isolation an antimicrobial action of endophytic fungi from sophora flavescens and effects on microorganism circumstances in soil. Lu He,Na Liu,Hongbo Xu,Na Yu,Yong Wang. 2013

[2]Antimicrobial action of an endophytic fungi from Sophor flavescens and structure identification of its active constituent. Yu, Na,He, Lu,Wang, Yong,Xu, Hongbo,Liu, Dandan,Liu, Na.

[3]Sequence analysis and heterologous expression of lectin-like gene CMLec3 from the medicinal fungus Cordyceps militaris. Bao, Da Peng,Ma, Yuan Wei,Gong, Ming,Li, Yan,Gao, Ying Nv,Yang, Rui Heng,Mao, Wen Jun,Wang, Ying,Yang, Rui Fang. 2018

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