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Characterization of a thermostable beta-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana

文献类型： 外文期刊

作者： Xu, Jing 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Zhu, Bo 1 ; Gao, Jian-Jie 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： Arabidopsis thaliana;beta-Glucuronidase;Thermostable enzyme;Thermotoga maritima

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2012 年 95 卷 5 期

页码：

收录情况： SCI

摘要： TmGUSI, a gene identical to that encoding a thermostable beta-glucuronidase in the hyperthermophilic anaerobe Thermotoga maritima, has been synthesized using a PCR-based two-step DNA synthesis and codon optimization for plants, and expressed in both Escherichia coli and Arabidopsis thaliana. TmGUSI expressed in transformed E. coli cells exhibited maximum hydrolytic activity at 65 A degrees C and pH 6.5 and retained more than 80% activity after incubation at 85 A degrees C for 30 min. TmGUSI activity in transgenic A. thaliana plants containing TmGUSI was also stable over the temperature range 65-80 A degrees C. Our data suggest that beta-glucuronidase from T. maritima can serve as a useful thermostable marker in higher plants.

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[1]High efficiency and throughput system in directed evolution in vitro of reporter gene. Xiong, Ai-Sheng,Peng, Ri-He,Liu, Jin-Ge,Zhuang, Jing,Qiao, Yu-Shan,Xu, Fang,Cai, Bing,Zhang, Zhen,Chen, Jian-Min,Yao, Quan-Hong.

[2]Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Li, Xian,Xue, Yong,Liu, Jin-Ge,Gao, Feng,Cai, Bin,Chen, Jian-Min,Yao, Quan-Hong.

[3]Directed evolution of beta-galactosidase from Escherichia coli into beta-glucuronidase. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Xu, Fang,Cai, Bin,Guo, Zhao-Kui,Qiao, Yu-Shan,Chen, Jian-Min,Zhang, Zhen,Yao, Quan-Hong. 2007

[4]Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability. Xiong, Ai-Sheng,Peng, Ri-He,Cheng, Zong-Ming,Li, Yi,Liu, Jin-Ge,Zhuang, Jing,Gao, Feng,Xu, Fang,Qiao, Yu-Shan,Zhang, Zhen,Chen, Jian-Min,Yao, Quan-Hong. 2007

[5]Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Zhuang, Jing,Yao, Quan-Hong,Sun, Chao-Cai,Zhou, Xi-Rong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min,Xiong, Fei.

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[11]A grape bHLH transcription factor gene, VvbHLH1, increases the accumulation of flavonoids and enhances salt and drought tolerance in transgenic Arabidopsis thaliana. Wang, Feibing,Li, Zhenjun,Peng, Rihe,Yao, Quanhong,Zhu, Hong,Chen, Dahu.

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