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Characterization of a thermostable beta-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana

文献类型： 外文期刊

作者： Xu, Jing 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Zhu, Bo 1 ; Gao, Jian-Jie 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： Arabidopsis thaliana;beta-Glucuronidase;Thermostable enzyme;Thermotoga maritima

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2012 年 95 卷 5 期

页码：

收录情况： SCI

摘要： TmGUSI, a gene identical to that encoding a thermostable beta-glucuronidase in the hyperthermophilic anaerobe Thermotoga maritima, has been synthesized using a PCR-based two-step DNA synthesis and codon optimization for plants, and expressed in both Escherichia coli and Arabidopsis thaliana. TmGUSI expressed in transformed E. coli cells exhibited maximum hydrolytic activity at 65 A degrees C and pH 6.5 and retained more than 80% activity after incubation at 85 A degrees C for 30 min. TmGUSI activity in transgenic A. thaliana plants containing TmGUSI was also stable over the temperature range 65-80 A degrees C. Our data suggest that beta-glucuronidase from T. maritima can serve as a useful thermostable marker in higher plants.

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