A Thermostable beta-Glucuronidase Obtained by Directed Evolution as a Reporter Gene in Transgenic Plants
文献类型： 外文期刊
作者： Xiong, Ai-Sheng 1 ; Peng, Ri-He 2 ; Zhuang, Jing 3 ; Chen, Jian-Min 4 ; Zhang, Bin 2 ; Zhang, Jian 3 ; Yao, Quan-Hong 2 ;
作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Coll Hort, Nanjing, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China
3.Alberta Innovates Technol Futures, Vegreville, AB, Canada
4.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2011 年 6 卷 11 期
页码：
收录情况： SCI
摘要： A beta-glucuronidase variant, GUS-TR3337, that was obtained by directed evolution exhibited higher thermostability than the wild-type enzyme, GUS-WT. In this study, the utility of GUS-TR337 as an improved reporter was evaluated. The corresponding gus-tr3337 and gus-wt genes were independently cloned in a plant expression vector and introduced into Arabidopsis thaliana. With 4-MUG as a substrate, plants containing the gus-wt gene showed no detectable b-glucuronidase activity after exposure to 60 degrees C for 10 min, while those hosting the gus-tr3337 gene retained 70% or 50% activity after exposure to 80 degrees C for 10 min or 30 min, respectively. Similarly, in vivo b-glucuronidase activity could be demonstrated by using X-GLUC as a substrate in transgenic Arabidopsis plants hosting the gus-tr3337 gene that were exposed to 80 degrees C for up to 30 min. Thus, the thermostability of GUS-TR3337 can be exploited to distinguish between endogenous and transgenic beta-glucuronidase activity, which is a welcome improvement in its use as a reporter.
- 相关文献
作者其他论文 更多>>
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
-
Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
作者：Wang, Bo;Wang, Ya-Hui;Deng, Yuan-Jie;Xiong, Ai-Sheng;Wang, Bo;Yao, Quan-Hong
关键词：Genetically modified carrot; Betanin; Unintended effects; Photosynthesis; Transcriptome; Metabolomics
-
Identification of Genes of Molecular Marker TGS0892 on Chromosome 6 and Its Mechanism of Soluble Solids Metabolism in Tomato
作者：Zhang, Rong-Rong;Tao, Jian-Ping;Zhang, Jia-Qi;Liu, Hui;Zhu, Wei-Min;Zhao, Tong-Min;Xiong, Ai-Sheng;Song, Liu-Xia;Zhao, Tong-Min;Zhu, Wei-Min
关键词：tomato; soluble solids; molecular marker; TGS0892; identification; gene expression
-
Complete biodegradation of the oldest organic herbicide 2,4-Dichlorophe- noxyacetic acid by engineering Escherichia coli
作者：Wang, Yu;Tian, Yong-Sheng;Gao, Jian-Jie;Xu, Jing;Li, Zhen-Jun;Fu, Xiao-Yan;Han, Hong-Juan;Wang, Li-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Qian, Cen;Zuo, Zhi-Hao;Wang, Bo;Peng, Ri-He;Yao, Quan-Hong
关键词：24-Dichlorophenoxyacetic acid; Biodegradation; Environmental pollution; Engineered bacteria; Synthetic biology
-
Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff
作者：Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong;Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong
关键词：recombinant enzymes; feed additive; feedstuff; nutrient utilization
