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Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China

文献类型: 外文期刊

作者: Zhang, Hui 1 ; Ma, Xin-ying 1 ; Qian, Ya-juan 1 ; Zhou, Xue-ping 1 ;

作者机构: 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr, Hangzhou 310029, Zhejiang, Peoples R China

2.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China

关键词: Papaya leaf curl China virus (PaLCuCNV);Tomato;Infectivity

期刊名称:JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B ( 影响因子:3.066; 五年影响因子:3.057 )

ISSN: 1673-1581

年卷期: 2010 年 11 卷 2 期

页码:

收录情况: SCI

摘要: Papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738-2751 nucleotides, which share 91.7%-97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-beta was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an infectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone.

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