Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China
文献类型: 外文期刊
作者: Zhang, Hui 1 ; Ma, Xin-ying 1 ; Qian, Ya-juan 1 ; Zhou, Xue-ping 1 ;
作者机构: 1.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr, Hangzhou 310029, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China
关键词: Papaya leaf curl China virus (PaLCuCNV);Tomato;Infectivity
期刊名称:JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B ( 影响因子:3.066; 五年影响因子:3.057 )
ISSN: 1673-1581
年卷期: 2010 年 11 卷 2 期
页码:
收录情况: SCI
摘要: Papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738-2751 nucleotides, which share 91.7%-97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-beta was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an infectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone.
- 相关文献
作者其他论文 更多>>
-
Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function
作者:Gong, Pan;Gao, Mengxin;Chen, Yalin;Zhang, Mingzhen;Hu, Xiaohua;Zhao, Siwen;Pan, Mengjiao;Cao, Buwei;Zhou, Xueping;Li, Fangfang;Gao, Mengxin;Zhou, Xueping;Huang, Yucong;Shen, Qingtang;Zhang, Hui;Liu, Yong;Liu, Yong;Lozano-Duran, Rosa;Wang, Aiming
关键词:positive-sense single-stranded RNA viruses; tobamoviruses; cucumber green mottle mosaic virus; small ORFs; peroxisomes; virus pathogenicity
-
Joint transcriptomics and metabolomics unveil the protective mechanism of tamarind seed polysaccharide against antibiotic-induced intestinal barrier damage
作者:Chen, Yinan;Sun, Xianbao;Kou, Yuxing;Ma, Xuan;Song, Lihua;Wu, Yan;Li, Xujiao;Zhang, Hui;Xie, Fan;Song, Zibo;Yuan, Chunmei;Huang, Siyan;Song, Zibo;Yuan, Chunmei;Huang, Siyan
关键词:Tamarind seed polysaccharide; Intestinal barrier damage; Transcriptomics; Metabolomics; Clindamycin
-
The role of multiple C2 domain and transmembrane region proteins in mediating tomato development
作者:Li, Siyi;Ling, Ribin;Liu, Lu;Xuan, Lijie;Li, Siyi;Wu, Xuexia;Zhang, Hui
关键词:Tomato; SlMCTP; Phylogenetic analysis; Hormone response; Main stem apex development
-
Effect of silver nanoparticles on biological nitrogen removal in sequential batch wastewater treatment process: Microbial communities, functional genes, and interactions
作者:Zhu, Yuliang;Zhang, Yan;Liu, Yucan;Zhang, Hui;Sun, Hongwei;Sun, Hongwei;Yang, Lei;Liu, Chengbin;Yang, Xinyi
关键词:Silver nanoparticles; Biological nitrogen removal; Co-occurrence networks; Interaction relationships; Functional genes
-
Overview of research on virus-resistant breeding of melon
作者:Tian, Shoubo;Diao, Qiannan;Cao, Yanyan;Yao, Dongwei;Zhang, Hui;Du, Xuan;Zhang, Yongping;Tian, Shoubo;Diao, Qiannan;Cao, Yanyan;Yao, Dongwei;Zhang, Hui;Du, Xuan;Zhang, Yongping;Zhang, Wenxian
关键词:virus- resistance;
Cucumis melo L.; QTL; MAS; sustainable agriculture -
Application of Exogenous Ascorbic Acid Enhances Cold Tolerance in Tomato Seedlings through Molecular and Physiological Responses
作者:Wang, Xinman;Ran, Chunxia;Fu, Yuandi;Han, Liyuan;Yang, Xuedong;Zhu, Weimin;Zhang, Hui;Zhang, Yingying
关键词:tomato; cold stress; ascorbic acid; seedlings; tolerance
-
Self-assembly of tamarind seed polysaccharide via enzymatic depolymerization and degalactosylation enhanced ice recrystallization inhibition activity
作者:Sun, Xianbao;Guo, Rui;Kou, Yuxing;Ma, Xuan;Song, Hong;Song, Lihua;Li, Xujiao;Wu, Yan;Zhan, Taijie;Zhang, Hui;Xie, Fan;Li, Xujiao;Song, Zibo;Yuan, Chunmei
关键词:Polysaccharide; Enzymatic modification; Self-assembly; Ice recrystallization inhibition; Cryoprotection; Molecular dynamics simulation



