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Pectic polysaccharides from Biluochun Tea: A comparative study in macromolecular characteristics, fine structures and radical scavenging activities in vitro

文献类型: 外文期刊

作者: Guo, Rui 1 ; Zhang, Jun'ai 1 ; Liu, Xin 1 ; Li, Xujiao 1 ; Sun, Xianbao 1 ; Kou, Yuxing 1 ; Li, Deshun 2 ; Liu, Yanfang 2 ; Zhang, Hui 3 ; Wu, Yan 1 ;

作者机构: 1.Shanghai Jiao Tong Univ, Shanghai Engn Res Ctr Food Safety, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Minist Agr,Natl R&D Ctr Edible Fungi Proc,Natl R&, Shanghai 201403, Peoples R China

3.Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Shanghai 200093, Peoples R China

关键词: Camellia sinensis L.; Biluochun Tea; Pectic polysaccharides; Macromolecular characteristics; Structural elucidations; Radical scavenging activities in vitro

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:8.025; 五年影响因子:7.626 )

ISSN: 0141-8130

年卷期: 2022 年 195 卷

页码:

收录情况: SCI

摘要: In this study, two acidic Biluochun Tea polysaccharides (BTP-A11 and BTP-A12) were investigated comparatively, which mainly consisted of Rha, Ara, Gal and GalA, possibly suggesting their pectic nature. Structurally, their galacturonan backbones composed of -> 4)-alpha-D-GalpA-(1 -> and -> 2)-alpha-L-Rhap-(1 -> were revealed similar, while Ara-and Gal-based branches attached to the O-2 of -> 2)-alpha-L-Rhap-(1 -> were in distinctive types, proportions, extensibilities and branching degrees. This could lead to their different macromolecular characteristics, where BTP-A(11) with higher M-w presented a more hyper-branched chain conformation and relatively higher structural flexibility/compactness, thereby resulting in a lower exclusion effect and an insufficient hydrodynamic volume. Besides, better radical scavenging activities in vitro were also determined for Gal-enriched BTP-A(11), where a larger surface area containing more H-donating groups were related to its higher Mw, more hyper-branched conformation, lower DM and higher DA. Therefore, the understanding of structure-property-activity relationships was improved to some degrees for acidic Biluochun Tea polysaccharides, which could be potentially required for more applications in food, medical and cosmetic fields.

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