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Ectopic expression of OsLFL1 in rice represses Ehdl by binding on its promoter

文献类型: 外文期刊

作者: Peng, Ling-Tao 1 ; Shi, Zheng-Ying 2 ; Li, Lin 2 ; Shen, Ge-Zhi 2 ; Zhang, Jing-Liu 3 ;

作者机构: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Shanghai Acad Agr Sci, Plant Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China

3.Chinese Acad Sci, Shanghai Ins

关键词: OsLFL1;RY cis-element;Ehdl;expression;rice

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

ISSN: 0006-291X

年卷期: 2007 年 360 卷 1 期

页码:

收录情况: SCI

摘要: 133 domain was identified as a novel DNA-binding motif specific to higher plant species. The B3 proteins play important roles in plant development. In the mutant W378, the mutant gene coding OsLFL1, a putative 133 transcription factor gene, was ectopically expressed. In this study, it was found that the flowering promoting gene Ehd1 and its putative downstream genes were all repressed by OsLFL1. Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) analyses suggest that OsLFL1 binds to the RY cis-elements (CATGCATG) in the promoter of the Ehd1 gene. Thus, ectopically expressed OsLFL1 might repress Ehd1 via binding directly to the RY cis-elements in its promoter. (c) 2007 Elsevier Inc. All rights reserved.

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