Agrobacterium-mediated transformation of the apple rootstock Malus micromalus Makino with the ROLC gene
文献类型： 外文期刊
作者： Zhang, Zhen 1 ; Sun, Aijun 2 ; Cong, Yu 2 ; Sheng, Bingcheng 2 ; Yao, Quanhong 2 ; Cheng, Zong-Ming 3 ;
作者机构： 1.Nanjing Agr Univ, Sch Hort, Nanjing 210095, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Sch Hort, Nanjing 210095, Jiangsu, Peoples R China; Shanghai Acad Agr Sci, Agrobio Res Ctr, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA
3.Nanjing Agr Univ, Sch Hort, Nanjing 210095, Jiangsu, Peoples R China; Shanghai Acad Agr Sci, Agrobio Res Ctr, Shanghai 201106, Peoples R China; Univ T
关键词： dwarf apple rootstock;genetic transformation
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
ISSN： 1054-5476
年卷期： 2006 年 42 卷 6 期
页码：
收录情况： SCI
摘要： Malus micromalus Makino was genetically engineered with the rolC gene via Agrobacterium-mediated transformation. The antibiotics carbenicillin, hygromycin, and kanamycin (Km) inhibited shoot regeneration front in, vitro leaf explants. The leaf segments were infected with Agrobacterium tumefaciens strain LBA4404 harboring one of the three different plasmids. Shoots were regenerated only from leaf segments infected with LBA4404 harboring the plasmid with the intron-containing neomycin phosphotransferase 11 gene as the selectable marker. Preculture of leaf segments on regeneration medium for 2 d before Agrobacterium treatment reduced formation of Km-resistant calluses. Transformation was confirmed by a histochemical beta-glucuronidase (GUS) assay, amplification of the rolC gene by polymerase chain reaction (PCR), and by Southern blot analysis. The rolC-transformed M. micromalus shoots showed an increased rooting ability without auxin treatment, and reduced height, internode length and leaf areas. This research shows the potential application of using the rolC gene for developing dwarf apple rootstocks.
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