Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum
文献类型： 外文期刊
作者： Zhang, HB 1 ; Zhang, DB 2 ; Chen, J 2 ; Yang, YD 2 ; Huang, ZJ 2 ; Huang, DF 2 ; Wang, XC 2 ; Huang, RF 2 ;
作者机构： 1.Chinese Acad Agr Sci, Natl Plant Gene Res Ctr Beijing, Beijing 100081, Peoples R China
2.Chinese Acad Agr Sci, Natl Plant Gene Res Ctr Beijing, Beijing 100081, Peoples R China; Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China; China Agr Univ, Coll Biol Sci, Natl Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Ctr, Shanghai 201106, Peoples R China; Chinese Acad Agr Sci, Vegetable & Flower Inst, Beijing 100081, Peoples R China
关键词： ethylene signaling;GCC box;PR gene expression;resistance to Ralstonia solanacearum;transient expression;TSRF1
期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）
ISSN： 0167-4412
年卷期： 2004 年 55 卷 6 期
页码：
收录情况： SCI
摘要： Ethylene responsive factors (ERFs) are important in regulating plant pathogen resistance, abiotic stress tolerance and plant development. Recent studies have greatly enlarged the ERF protein family and revealed more important roles of ERFs in plants. Here, we report our finding of a tomato ERF protein TSRF1, which is transcriptionally up-regulated by ethylene, salicylic acid, or Ralstonia solanacearum strain BJ1057 infection. Biochemical analysis indicates that TSRF1 specifically interacts in vitro with the GCC box, an element present in the promoters of many pathogenesis-related (PR) genes. Further investigation evidences that TSRF1 activates in vivo the expression of reporter beta-glucuronidase gene controlled by GCC box. More importantly, overexpressing TSRF1 in tobacco and tomato constitutively activates the expression of PR genes, and subsequently enhancing transgenic plant resistance to the bacterial wilt caused by Ralstonia solanacearum strain BJ1057. Therefore our investigation not only extends the functions of ERF proteins in plant resistance to R. solanacearum, but also provides further clues to understanding the mechanism of host regulatory proteins in response to the infection of pathogens.
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