Multiplex polymerase chain reaction method for detection of bovine materials in foodstuffs
文献类型： 外文期刊
作者： Gao, HW 1 ; Zhang, DB 2 ; Pan, AH 2 ; Liang, WQ 2 ; Liang, CZ 2 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotech Res Ctr, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotech Res Ctr, Shanghai, Peoples R China; Qingdao Engry Exit Inspect & Quarantine Bur, Dept Food Adm, Qingdao, Peoples R China
期刊名称：JOURNAL OF AOAC INTERNATIONAL （ 影响因子：1.913； 五年影响因子：1.754 ）
ISSN： 1060-3271
年卷期： 2003 年 86 卷 4 期
页码：
收录情况： SCI
摘要： Rapid identification of bovine materials in animal foodstuffs is essential for effective control of a potential source of bovine spongiform encephalophathy. A convenient polymerase chain reaction (PCR)-based assay was developed for detection and identification of a bovine-specific genomic DNA sequence in foodstuffs. Simultaneously the assay assessed the DNA quality of the experiment system by amplification of a highly conserved eucaryotic DNA region of the 18-S ribosomal gene, helping to check the reliability of the test result. The amplified bovine-specific PCR product was a genomic DNA fragment of lactoferrin, a low copy gene that was different from a commonly used bovine-specific mitochondria sequence for identification of bovine materials. The specificity of this method was confirmed by the absence of detectable homologous PCR product using reference foodstuff samples that lacked bovine-derived meat and bonemeals, or genomic DNA samples from vertebrates whose offals are commonly included in animal feeds. This method could detect the presence of bovine material in foodstuffs when the samples contained >0.02% bovine-derived meat and bone meal. Furthermore, it was not affected by prolonged heat treatment. The specificity, convenience, and sensitivity of this method suggest that it can be used for the routine detection of bovine-derived materials.
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