您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Environmentally relevant concentrations of mercury facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes

文献类型： 外文期刊

作者： Li, Wen 1 ; Zhang, Wei-Guo 1 ; Zhang, Ming-Sha 6 ; Lei, Zhong-Fang 4 ; Li, Peng-Fu 2 ; Ma, Yan 3 ; Gao, Yan 3 ;

作者机构： 1.Minist Sci & Technol, Jiangsu Key Lab Food Qual, Safety State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

2.Nanjing Univ, Sch Life Sci, Nanjing 210093, Peoples R China

3.Inst Agr Resources & Environm, Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

4.Univ Tsukuba, Sch Life & Environm Sci, Tsukuba 3058577, Japan

5.China Minist Agr, Key Lab Yangtze River Plain Agr Environm, Nanjing 210014, Peoples R China

6.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China

7.Natl Agr Expt Stn Agricultrual Environm, Nanjing 210014, Peoples R China

关键词： Antibiotic resistance genes; Mercury; Conjugative transfer; Public health

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2022 年 852 卷

页码：

收录情况： SCI

摘要： Abundant antibiotic resistance genes (ARGs) are typically found in mercury (Hg)-contaminated aquatic environments. This phenomenon is partly attributed to the co-resistance, cross-resistance, and shared regulatory responses to Hg and antibiotics. However, it remains unclear whether and how Hg influences the conjugative transfer of ARGs mediated by mobilizable plasmids. In the present study, we found that Hg2+ at the environmentally relevant concentrations (0.001-0.5 mg L-1) facilitated the conjugative transfer of ARGs through the mobilizable plasmid RP4 from the donor Escherichia coli HB101 to the recipient E. coli K12. Exposure to Hg2+ significantly increases the formation of reactive oxygen species, malondialdehyde production, antioxidant enzyme activities, and cell membrane permeability, while decreasing the concentration of glutathione. Scanning electron microscopy and transmission electron micros-copy showed that the cell membrane suffered from oxidative damage, which is beneficial for conjugative transfer. The expression of global regulatory genes (korA, korB, and trbA) negatively regulating conjugative transfer was restrained by Hg2+, while promoting the expression of positive regulatory genes involved in the mating pair formation system (trbBp and traF) and the DNA transfer and replication systems (trfAp and traJ). Although a high Hg2+ concentration (1.0 mg L-1) suppressed ARGs conjugative transfer, our results suggest that Hg2+ facilitates the dissemination of ARGs in aquatic environments at environmentally relevant concentrations. This study improves our understanding of ARGs dissemination in Hg-contaminated aquatic environments.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台